| Author | Medeiros, Igor Dias | |
| Author | Siebert, Marília Nardelli | |
| Author | Silva, Guilherme de Toledo e | |
| Author | Moraes, Milton Ozório | |
| Author | Marques, Maria Risoleta Freire | |
| Author | Bainy, Afonso Celso Dias | |
| Access date | 2019-03-07T14:17:46Z | |
| Available date | 2019-03-07T14:17:46Z | |
| Document date | 2008 | |
| Citation | MEDEIROS, Igor Dias et al. Differential gene expression in oyster exposed to sewage. Marine Environmental Research, v. 66, p. 156-157, July 2008. | |
| ISSN | 0141-1136 | |
| URI | https://www.arca.fiocruz.br/handle/icict/31970 | |
| Description | Produção científica do Laboratório de Hanseníase. | pt_BR |
| Sponsorship | This work was supported by the Brazilian Council for the Scientific and Technological Development (CNPQ), Grant No. 475995/2003-1. | |
| Sponsorship | Afonso Celso Dias Bainy is recipient of the CNPQ productivity fellowship. | |
| Language | eng | en_US |
| Publisher | Elsevier | |
| Rights | restricted access | |
| Title | Differential gene expression in oyster exposed to sewage | en_US |
| Type | Article | |
| DOI | 10.1016/j.marenvres.2008.02.048 | |
| Abstract | In order to investigate the influence of domestic sewage on the gene expression of Pacific oysters Crassostrea gigas, suppression subtractive hybridization (SSH) method was employed. Oysters were sampled at a farming area and, after 10 days of acclimation in the laboratory, were exposed to untreated domestic sewage diluted 33% for 48 h. Gills of male oysters were excised for total RNA extraction. mRNA was purified and the differential gene expression was analyzed by SSH. We obtained 61 cDNA sequences but only 15 were identified, which includes fatty acid binding protein, multidrug resistance protein, omega glutathione S-transferase, cytochrome P450 isoform CYP356A1, among others. The identified genes are associated with different metabolic functions like biotransformation, membrane transport, aerobic metabolism and translational machinery, evidencing the potential toxic effects elicited by these effluents. | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Centro de Ciências Biológicas. Departamento de Bioquímica. Florianópolis, SC, Brasil / Universidade do Sul de Santa Catarina. Laboratório de Ciências Marinhas. Laguna, SC, Brasil. | |
| Affilliation | Universidade Federal de Santa Catarina. Centro de Ciências Biológicas. Departamento de Bioquímica. Florianópolis, SC, Brasil. | |
| Affilliation | Universidade Federal de Santa Catarina. Centro de Ciências Biológicas. Departamento de Bioquímica. Florianópolis, SC, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal de Santa Catarina. Centro de Ciências Biológicas. Departamento de Bioquímica. Florianópolis, SC, Brasil. | |
| Affilliation | Universidade Federal de Santa Catarina. Centro de Ciências Biológicas. Departamento de Bioquímica. Florianópolis, SC, Brasil. | |
| Subject | Crassostrea gigas | en_US |
| Subject | SSH | en_US |
| Subject | Gene expression | en_US |
| Subject | Sewage | en_US |
| Subject | Biomarker | en_US |
| Subject | Monitoring | en_US |
| e-ISSN | 1879-0291 | |
| xmlui.metadata.dc.subject.ods | 06 Água potável e saneamento | |
