| Author | Godinho, Rodrigo Maciel da Costa | |
| Author | Crestani, Juliana | |
| Author | Kmetzsch, Lívia | |
| Author | Araujo, Glauber de S. | |
| Author | Frases, Susana | |
| Author | Staats, Charley C. | |
| Author | Schrank, Augusto | |
| Author | Vainstein, Marilene Henning | |
| Author | Rodrigues, Marcio Lourenço | |
| Access date | 2019-03-07T19:09:52Z | |
| Available date | 2019-03-07T19:09:52Z | |
| Document date | 2014 | |
| Citation | GODINHO, R. M. et al. The vacuolar-sorting protein Snf7 is required for export of virulence determinants in members of the Cryptococcus neoformans complex. Scientific Reports, v. 4, p. 1-11, 2014. | pt_BR |
| ISSN | 2045-2322 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/31990 | |
| Language | eng | pt_BR |
| Publisher | Nature | pt_BR |
| Rights | open access | pt_BR |
| Title | The vacuolar-sorting protein Snf7 is required for export of virulence determinants in members of the Cryptococcus neoformans complex | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1038/srep06198 | |
| Abstract | Fungal pathogenesis requires a number of extracellularly released virulence factors. Recent studies demonstrating that most fungal extracellular molecules lack secretory tags suggest that unconventional secretion mechanisms and fungal virulence are strictly connected. Proteins of the endosomal sorting complex required for transport (ESCRT) have been recently associated with polysaccharide export in the yeast-like human pathogen Cryptococcus neoformans. Snf7 is a key ESCRT operator required for unconventional secretion in Eukaryotes. In this study we generated snf7Δ mutant strains of C. neoformans and its sibling species C. gattii. Lack of Snf7 resulted in important alterations in polysaccharide secretion, capsular formation and pigmentation. This phenotype culminated with loss of virulence in an intranasal model of murine infection in both species. Our data support the notion that Snf7 expression regulates virulence in C. neoformans and C. gattii by ablating polysaccharide and melanin traffic. These results are in agreement with the observation that unconventional secretion is essential for cryptococcal pathogenesis and strongly suggest the occurrence of still obscure mechanisms of exportation of non-protein molecules in Eukaryotes. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Metrologia, Normalização e Qualidade Industrial. Laboratório de Biologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Metrologia, Normalização e Qualidade Industrial. Laboratório de Biologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Departamento de Biologia Molecular e Biotecnologia. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Departamento de Biologia Molecular e Biotecnologia. Porto Alegre, RS, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Vacuolar-sorting protein Snf7 | pt_BR |
| Subject | Virulence determinants | pt_BR |
| Subject | Cryptococcus neoformans complex | pt_BR |
