| Author | Andrade, Hélida M. | |
| Author | Murta, Silvane M. F. | |
| Author | Chapeaurouge, Alex | |
| Author | Perales, Jonas | |
| Author | Nirdé, Phillipe | |
| Author | Romanha, Alvaro J. | |
| Access date | 2019-03-14T11:50:25Z | |
| Available date | 2019-03-14T11:50:25Z | |
| Document date | 2008 | |
| Citation | ANDRADE, Hélida M. et al. Proteomic Analysis of Trypanosoma cruzi Resistance to Benznidazole. Journal of Proteome Research, v.7, p.2357–2367, May 2008. | pt_BR |
| ISSN | 1535-3893 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/32068 | |
| Language | eng | pt_BR |
| Publisher | American Chemical Society | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Benzonidazol | pt_BR |
| Subject in Portuguese | Proteoma | pt_BR |
| Subject in Portuguese | Resistência | pt_BR |
| Title | Proteomic analysis of Trypanosoma cruzi resistance to Benznidazole | pt_BR |
| Type | Article | |
| DOI | 10.1021/pr700659m | |
| Abstract | The first proteomic analysis of Trypanosoma cruzi resistance to Benznidazole (BZ) is presented. The differential proteome of T. cruzi with selected in vivo resistance to Benznidazole (BZR and Clone27R), its susceptible pairs (BZS and Clone9S), and a pair from a population with Benznidazole- in vitro-induced resistance (17LER) and the susceptible pair 17WTS were analyzed by two-dimensional gel electrophoresis (2-DE) followed by mass spectrometry (MS) for protein identification. Out of 137 spots analyzed through MS, 110 were identified as 56 distinct proteins. Out of the 56 distinct proteins, 36 were present in resistant, 9 in susceptible, and 11 in both phenotypes. Among the proteins identified in resistant samples, 5 were found in Cl 27R and in BZR (calpain-like cysteine peptidase, hypothetical protein conserved 26 kDa, putative peptidase, peroxiredoxin and tyrosine amino transferase) and 4 in Cl 27R and 17LER (cyclophilin A, glutamate dehydrogenase, iron superoxide dismutase and nucleoside diphosphate kinase). As for the proteins identified in Benznidazole-susceptible samples, PGF-2a was found in BZS and 17WTS. A functional category analysis showed that the proteins involved with transcription and protein destination were overexpressed for the Benznidazole-resistant phenotype. Thus, the present study provides large-scale, protein-related information for investigation of the mechanism of T. cruzi resistance to Benznidazole. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Imunoparasitologia. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil / Universidade Federal do Piauí. PI, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | INSERM U540. Montpellier, France. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Benznidazole | pt_BR |
| Subject | Resistance | pt_BR |
| Subject | Proteome | pt_BR |
| Subject | Electrophoresis, Gel, Two-Dimensional | pt_BR |
| e-ISSN | 1535-3907 | |
| Embargo date | 2022-01-01 | |
