| Author | Marín-Villa, Marcel | |
| Author | Vargas-Inchaustegui, Diego A. | |
| Author | Chaves, Suzana P. | |
| Author | Tempone, Antonio J. | |
| Author | Dutra, Juliana M. F. | |
| Author | Soares, Maurilio J. | |
| Author | Ueda-Nakamura, Tania | |
| Author | Mendonça, Sergio C. F. | |
| Author | Rossi-Bergmann, Bartira | |
| Author | Soong, Lynn | |
| Author | Traub-Csekö, Yara M. | |
| Access date | 2019-03-14T12:15:54Z | |
| Available date | 2019-03-14T12:15:54Z | |
| Document date | 2008 | |
| Citation | MARÍN-VILLA, Marcel; et al. The C-terminal extension of Leishmania pifanoi amastigote-specific cysteine proteinase Lpcys2: A putative function in macrophage infection. Molecular & Biochemical Parasitology, v.162, p.52–59, 2008. | pt_BR |
| ISSN | 0166-6851 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/32071 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Leishmania | pt_BR |
| Subject in Portuguese | Macrófagos | pt_BR |
| Subject in Portuguese | Infecção | pt_BR |
| Subject in Portuguese | Proteinase cisteína | pt_BR |
| Subject in Portuguese | Extensão C-terminal | pt_BR |
| Title | The C-terminal extension of Leishmania pifanoi amastigote-specific cysteine proteinase Lpcys2: a putative function in macrophage infection | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.molbiopara.2008.07.003 | |
| Abstract | Cysteine proteinases have been implicated in many aspects of protozoan parasite pathogenesis. These hydrolases are normally found as zymogens, and some classes in trypanosomatids possess a long C-terminal extension (CTE), for which no function has been assigned. In this paper we hypothesize that the CTE domain of Lpcys2, the abundant lysosomal cysteine proteinase of Leishmania pifanoi amastigotes, is involved in host cell infection. Confirming previous reports that this peptide is highly immunogenic in Trypanosoma cruzi, we detected antibodies against CTE in sera of leishmaniasis patients. We produced a polyclonal antibody specific to Lpcys2 CTE and determined that this antibody was capable of recognizing both L. pifanoi and Leishmania amazonensis cysteine proteinases. Using this antibody, we detected a predominant localization of Lpcys2 CTE in the lysosome and flagellar pocket of cultured axenic amastigotes of both parasite species; however, its location was shifted towards the surface of the parasites during macrophage infection. We examined the role of Lpcys2 CTE in macrophage infection and found a significant reduction in the percentage of infected cells when macrophages were infected with L. pifanoi and L. amazonensis in the presence of anti-CTE antibody. This study suggests a role for leishmanial cysteine proteinases CTE at early stages of infection. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Tripanosomatídeos e Flebotomíneos. Rio de Janeiro, RJ. Brasil / Universidad de Antioquia - PECET. Medellín, Colombia. | pt_BR |
| Affilliation | The University of Texas Medical Branch. Department of Microbiology and Immunology. Galveston, TX, USA. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Tripanosomatídeos e Flebotomíneos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Tripanosomatídeos e Flebotomíneos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Estadual de Maringá. Centro de Ciências da Saúde. Departamento de Análises Clínicas. Maringá, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | The University of Texas Medical Branch. Department of Microbiology and Immunology. Galveston, TX, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Tripanosomatídeos e Flebotomíneos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Leishmania | pt_BR |
| Subject | Macrophage | pt_BR |
| Subject | Cysteine proteinase | pt_BR |
| Subject | C-terminal extension | pt_BR |
| Subject | Infection | pt_BR |
| e-ISSN | 1872-9428 | |
| Embargo date | 2022-01-01 | |
