| Author | Paiva, Luciana S. de | |
| Author | Nobrega, Alberto | |
| Author | Melo, Giany O. de | |
| Author | Hayashi, Elize A. | |
| Author | Carvalho, Vinicius | |
| Author | Silva, Patricia M. Rodrigues e | |
| Author | Bellio, Maria | |
| Author | Teixeira, Gerlinde P. | |
| Author | Rumjanek, Vivian | |
| Author | Costa, Sonia S. | |
| Author | Koatz, Vera Lúcia G. | |
| Access date | 2019-03-19T11:32:42Z | |
| Available date | 2019-03-19T11:32:42Z | |
| Document date | 2008 | |
| Citation | PAIVA, Luciana S. de et al. Selective blockade of lymphopoiesis induced by kalanchosine dimalate: inhibition of IL-7-dependent proliferation. Journal of Leukocyte Biology, v. 83, p. 1038-1048, Apr. 2008. | pt_BR |
| ISSN | 0741-5400 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/32140 | |
| Language | eng | pt_BR |
| Publisher | Society for Leukocyte Biology | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Kalanchoe brasiliensis | pt_BR |
| Subject in Portuguese | Corticosteróides | pt_BR |
| Subject in Portuguese | Interferon | pt_BR |
| Title | Selective blockade of lymphopoiesis induced by kalanchosine dimalate: inhibition of IL-7-dependent proliferation | pt_BR |
| Type | Article | |
| DOI | 10.1189/jlb.0707441 | |
| Abstract | Lymphopoiesis and myelopoiesis continuously generate mature cells from hematopoietic cell progenitors during the lifetime of the organism. The identification of new endogenous or exogenous substances that can act specifically on the differentiation of distinct cell lineages is of relevance and has potential therapeutical use. Kalanchoe brasiliensis (Kb) is a medicinal plant from the Crassulaceae family, used in folk medicine to treat inflammatory and infectious diseases. Here, we show that short-term treatment of naïve mice with Kb led to a strong and selective inhibition of lymphopoiesis, affecting B and T cell lineages without reduction of the myeloid lineage development. Similar effects were observed after treatment with the highly purified compound kalanchosine dimalate (KMC), obtained from Kb. Numbers of mature lymphocytes in secondary lymphoid organs were preserved in Kb(KMC)-treated mice. The effect of Kb(KMC) was not a result of secondary augmentation of plasma levels of endogenous corticoids; neither involves TNF-alpha, type-I IFN, or TLR2/TLR4 ligands, which have all been described as selective inhibitors of lymphopoiesis. Flow cytometry analysis of the phenotypes of T and B cell precursors indicate a blockade of maturation on IL-7-dependent, proliferative stages. In vitro, Kb(KMC) inhibited the IL-7-dependent proliferation of pre-B cells and does not induce massive apoptosis of B and T cell precursors. These results suggest that Kb(KMC) is selectively blocking lymphopoiesis through a mechanism that does not involve the previously characterized substances, possibly acting on the IL-7 signaling pathway, opening new perspectives for a potential therapeutic use of Kb-derived drugs. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Núcleo de Pesquisas de Produtos Naturais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto de Biologia. Departamento de Imunobiologia. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Núcleo de Pesquisas de Produtos Naturais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Kalanchoe brasiliensis | pt_BR |
| Subject | corticosteroids | pt_BR |
| Subject | TLR | pt_BR |
| Subject | IFN | pt_BR |
| Subject | TNF | pt_BR |
| DeCS | Fator de Necrose Tumoral | pt_BR |
| e-ISSN | 1938-3673 | |
| Embargo date | 2022-01-01 | |
