| Author | Campos, Rodrigo M. | |
| Author | Nascimento, Mirna | |
| Author | Ferraz, J. Cândido | |
| Author | Pereira, Mariana M. C. | |
| Author | Rocha, Pollyanna O. | |
| Author | Thompson, Glória M. | |
| Author | Cysne-Finkelstein, Léa | |
| Author | Figueiredo, Regina C. B. Q. | |
| Author | Melo Neto, Osvaldo P. de | |
| Access date | 2019-03-19T17:11:28Z | |
| Available date | 2019-03-19T17:11:28Z | |
| Document date | 2008 | |
| Citation | CAMPOS, Rodrigo M. et al. Distinct mitochondrial HSP70 homologues conserved in various Leishmania species suggest novel biological functions. Molecular & Biochemical Parasitology, v.160, p.157–162, 2008. | pt_BR |
| ISSN | 0166-6851 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/32160 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Leishmania chagasi | pt_BR |
| Subject in Portuguese | Proteínas de choque térmico | pt_BR |
| Subject in Portuguese | Mitocôndria | pt_BR |
| Title | Distinct mitochondrial HSP70 homologues conserved in various Leishmania species suggest novel biological functions | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.molbiopara.2008.04.013 | |
| Abstract | We report the identification of two distinct homologues of the 70-kDa mitochondrial heat shock protein (mtHSP70) from Leishmania chagasi/Leishmania infantum (Lc2.1 and Lc2.2). In Leishmania species, multiple genes encoding Lc2.2 are present whilst single genes encode Lc2.1. Strikingly, genes encoding Lc2.1-like proteins are absent from Trypanosoma species. Lc2.2 is characterized by a poly-glutamine rich C-terminus, absent from Lc2.1 or mtHSP70 homologues outside the trypanosomatids. Lc2.1 displays unique substitutions within its peptide-binding domain which modify amino acids strictly conserved in cytoplasmic and mitochondrial HSP70 proteins alike. Affinity purified antibodies recognize mainly a single protein in extracts from promastigotes/epimastigotes of various Leishmania/Trypanosoma species. Upon differentiation of Leishmania amazonensis into amastigotes a second protein (presumably Lc2.1) is induced and becomes the predominant mtHSP70 homologue expressed. Subcellular localization of these proteins was investigated and ratified a distribution throughout the mitochondrial matrix. Our results imply novel mtHSP70 functions which evolved within the genus Leishmania. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | McGill University. Department of Microbiology and Immunology. 3775 University Street, Lyman-Duff Building, Montreal, Canada. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Centro Acadêmico de Vitória. Vitória de Santo Antão, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade do Estado de São Paulo. Escola de Odontologia de Araraquara. Departamento de Diagnóstico e Cirurgia. Araraquara, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Subject | Leishmania chagasi | pt_BR |
| Subject | Heat shock proteins | pt_BR |
| Subject | mtHSP70 | pt_BR |
| Subject | Mitochondria | pt_BR |
| e-ISSN | 1872-9428 | |
| Embargo date | 2022-01-01 | |
