Author | Cardoso, Josiane | |
Author | Soares, Maurilio José | |
Author | Barreto, Rubem Figueiredo Sadok Menna | |
Author | Le Bloas, Rozenn | |
Author | Sotomaior, Vanessa Santos | |
Author | Goldenberg, Samuel | |
Author | Krieger, Marco Aurelio | |
Access date | 2019-03-20T11:37:31Z | |
Available date | 2019-03-20T11:37:31Z | |
Document date | 2008 | |
Citation | CARDOSO, Josiane et al. Inhibition of proteasome activity blocks Trypanosoma cruzi growth and metacyclogenesis. Parasitology Research, v. 103, p. 941-951, 26 June 2008. | |
ISSN | 0932-0113 | |
URI | https://www.arca.fiocruz.br/handle/icict/32167 | |
Description | Produção científica do Laboratório de Biologia Celular. | pt_BR |
Language | eng | en_US |
Publisher | Springer | |
Rights | restricted access | |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Subject in Portuguese | Crescimento | pt_BR |
Subject in Portuguese | Metaciclogênese | pt_BR |
Subject in Portuguese | Inibidores do proteassoma | pt_BR |
Title | Inhibition of proteasome activity blocks Trypanosoma cruzi growth and metacyclogenesis | en_US |
Type | Article | |
DOI | 10.1007/s00436-008-1081-6 | |
Abstract | Proteasomes are intracellular complexes that control protein degradation in organisms ranging from Archaebacteria to mammals. In some parasitic protozoa, the proteasome is involved in cell differentiation and replication. In this study, we have used proteasome inhibitors to determine the biological role of proteasomes during the replication and in vitro metacyclogenesis of Trypanosoma cruzi. We used light and transmission electron microscopy to analyze morphological data and flow cytometry to analyze changes in the cell cycle. The growth of T. cruzi epimastigote culture forms in liver infusion tryptose medium was inhibited by the presence of up to 10 microM lactacystin. Inhibition was dose-dependent, with IC50 (50% inhibitory concentration) of 4.35 microM after 24 or 72 h. The metacyclogenesis process in vitro was strongly (95%) inhibited by 5 microM lactacystin treatment. The adhesion phase was not affected, but the epimastigotes did not differentiate into metacyclic trypomastigotes. Most treated epimastigotes had replicated DNA, with swelling of the mitochondrion and an altered distribution of nuclear and kinetoplast DNA. Our findings suggest that inhibition of the ubiquitin-proteasome pathway in T. cruzi epimastigotes does not block adhesion, but disrupts cell division and affects factors triggering differentiation. | en_US |
Affilliation | Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | |
Affilliation | Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | |
Affilliation | Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil / Pontifícia Universidade Católica do Paraná. Curitiba, PR, Brasil. | |
Affilliation | Pontificia Universidade Católica do Paraná. Curitiba, PR, Brasil. | |
Affilliation | Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | |
Affilliation | Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | |
Subject | Trypanosoma cruzi | en_US |
Subject | Proteasome inhibitors | en_US |
Subject | Metacyclogenesis | en_US |
Subject | Growth | en_US |
e-ISSN | 1432-1955 | |