| Author | Silva, Guilherme de Toledo | |
| Author | Siebert, Marília Nardelli | |
| Author | Medeiros, Igor Dias | |
| Author | Sincero, Thaís Cristine Marques | |
| Author | Moraes, Milton Ozório | |
| Author | Goldstone, Jared V. | |
| Author | Bainy, Afonso Celso Dias | |
| Access date | 2019-03-20T12:54:31Z | |
| Available date | 2019-03-20T12:54:31Z | |
| Document date | 2008 | |
| Citation | SILVA, Guilherme de Toledo et al. Cloning a new cytochrome P450 isoform (CYP356A1) from oyster Crassostrea gigas. Marine Environmental Research, v. 66, p. 15-18, July 2008. | |
| ISSN | 0141-1136 | |
| URI | https://www.arca.fiocruz.br/handle/icict/32171 | |
| Description | Produção científica do Laboratório de Hanseníase. | pt_BR |
| Sponsorship | This work was supported by CNPq-Universal (Grant #484328/2006–9) to Afonso Celso Dias Bainy. | |
| Sponsorship | Afonso Celso Dias Bainy is recipient of Productivity Fellowship from CNPq. | |
| Language | eng | en_US |
| Publisher | Elsevier | |
| Rights | restricted access | |
| Title | Cloning a new cytochrome P450 isoform (CYP356A1) from oyster Crassostrea gigas | en_US |
| Type | Article | |
| DOI | 10.1016/j.marenvres.2008.02.010 | |
| Abstract | We have cloned the full-length cDNA of the first member of a new cytochrome P450 (CYP) family from the Pacific oyster Crassostrea gigas. This new CYP gene was obtained based on an initial 331 bp fragment previously identified among the list of the differentially expressed genes in oysters exposed to untreated domestic sewage. The full-length CYP has an open reading frame of 1500 bp and based on its deduced amino acid sequence was classified as a member of a new subfamily, CYP356A1. A phylogenetic analysis showed that CYP356A1 is closely related to members of the CYP17 and CYP1 subfamilies. Semi-quantitative RT-PCR was performed to analyze the CYP356A1 expression in different tissues of the oyster (digestive gland, gill, mantle and adductor muscle). Results showed slightly higher CYP356A1 expression in digestive gland and mantle, than the other tissues, indicating a possible role of the CYP356A1 in xenobiotic biotransformation and/or steroid metabolism. | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Bioquímica. Laboratório de Biomarcadores de Contaminação Aquática. Florianópolis, SC, Brasil. | |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Bioquímica. Laboratório de Biomarcadores de Contaminação Aquática. Florianópolis, SC, Brasil. | |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Bioquímica. Laboratório de Biomarcadores de Contaminação Aquática. Florianópolis, SC, Brasil / Universidade do Sul de Santa Catarina. Laboratório de Ciências Marinhas. Laguna, SC, Brasil. | |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Laboratório de Protozoologia. Florianópolis, SC, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Woods Hole Oceanographic Institution. Biology Department. Woods Hole, MA, USA. | |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Bioquímica. Laboratório de Biomarcadores de Contaminação Aquática. Florianópolis, SC, Brasil. | |
| Subject | Cytochrome P450 | en_US |
| Subject | Pacific oyster | en_US |
| Subject | Crassostrea gigas | en_US |
| Subject | Biotransformation enzymes | en_US |
| Subject | CYP356A1 | en_US |
| Subject | CYP17 | en_US |
| e-ISSN | 1879-0291 | |
