| Author | Rocha, Natália Pereira da | |
| Author | Avvad-Portari, Elyzabeth | |
| Author | Russomano, Fábio | |
| Author | Roma, Eric Henrique | |
| Author | Pinto, Amanda Chaves | |
| Author | Klumb, Evandro | |
| Author | Macedo, Jacyara | |
| Author | Fernandes, Ana Teresa Gomes | |
| Author | Bonecini-Almeida, Maria da Glória | |
| Access date | 2019-03-22T19:36:22Z | |
| Available date | 2019-03-22T19:36:22Z | |
| Document date | 2019 | |
| Citation | ROCHA, Natália Pereira da et al. TNFR1 single nucleotide polymorphisms are not associated with cervical HPV-induced pre-malignant lesion but regulate in situ cervical TNFR1 expression. Oncotarget, v. 10, n. 9, p. 953-965, 2019. | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/32208 | |
| Language | eng | pt_BR |
| Publisher | Impact Journals | pt_BR |
| Rights | open access | |
| Title | TNFR1 single nucleotide polymorphisms are not associated with cervical HPV-induced pre-malignant lesion but regulate in situ cervical TNFR1 expression | pt_BR |
| Type | Article | pt_BR |
| Abstract | TNF-α is involved in HPV infection control by triggering cell signaling through binding in specific receptors TNFR1 and TNFR2. Genetic polymorphisms in these receptors may influence TNF-α signaling. Herein, we investigated TNFR1 rs767455 andrs2234649 single nucleotide polymorphisms, and TNFR1 protein expression in cervical squamous intraepithelial lesions (SIL) to identify their role in cervical pre-malignant development. SIL patients (n = 179) and healthy volunteers (n = 227) were enrolled for TNFR1 genotyping analysis by PCR-RFLP in blood samples and TNFR1 protein expression in cervical tissue by immunohistochemistry. No statistical differences regard genotypes and allelic frequencies for both polymorphisms were observed.
Cervical TNFR1-expressing cells were rare in epithelium and basal layer regardless the groups. However, a progressive increase in infiltrating cells was observed in the stromal area, mainly in high SIL (HSIL) group compared to low SIL (LSIL, p <0.001) and control (p < 0.001) groups. TNFR1-expressing cells frequency was higher in TNFR1 rs767455AG/GG (p < 0.001), and in rs2234649AA (p < 0.001) genotypes carries in HSIL subgroup. These data indicated that TNFR1-expression is abrogated in cervical epithelium, where HPV-induced pre-malignant lesion occurs, increasing its frequency in inflammatory cells in stroma, and is genetically controlled by TNFR1 rs767455AG/GG and rs234649AA genotypes. These biomarkers may be useful to identify cervical precancerous lesions progression. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Imunologia e Imunogenética em Doenças Infecciosas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Saúde da Mulher, da Criança e do Adolescente Fernandes Figueira. Laboratório de Anatomia Patológica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Saúde da Mulher, da Criança e do Adolescente Fernandes Figueira. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Imunologia e Imunogenética em Doenças Infecciosas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | State University of Rio de Janeiro. Department of Biochemistry. Rio de Janeiro, RJ, Brazil. | pt_BR |
| Affilliation | State University of Rio de Janeiro. Department of Rheumatology. Rio de Janeiro, RJ, Brazil. | pt_BR |
| Affilliation | State University of Rio de Janeiro. Department of Biochemistry. Rio de Janeiro, RJ, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Imunologia e Imunogenética em Doenças Infecciosas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Imunologia e Imunogenética em Doenças Infecciosas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | HPV | pt_BR |
| Subject | Cervical lesions | pt_BR |
| Subject | In situ TNFR1 | pt_BR |
| Subject | TNFR1 SNPs | pt_BR |
| e-ISSN | 1949-2553 | |
