| Author | Pitaluga, A. N. | |
| Author | Mason, P. W. | |
| Author | Traub-Cseko, Y. M. | |
| Access date | 2019-03-24T15:16:20Z | |
| Available date | 2019-03-24T15:16:20Z | |
| Document date | 2008 | |
| Citation | PITALUGA, A. N.; MASON, P. W.; TRAUB-CSEKO, Y. M. Non-specific antiviral response detected in RNA-treated cultured cells of the sandfly, Lutzomyia longipalpis. Developmental and Comparative Immunology, v. 32, p. 191-197, 2008. | pt_BR |
| ISSN | 0145-305X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/32213 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Lutzomyia Longipalpis | pt_BR |
| Subject in Portuguese | Resposta antiviral | pt_BR |
| Subject in Portuguese | Vírus do Nilo Ocidental | pt_BR |
| Subject in Portuguese | Partícula semelhante a vírus | pt_BR |
| Subject in Portuguese | Imunidade inata | pt_BR |
| Subject in Portuguese | Silenciamento de genes | pt_BR |
| Title | Non-specific antiviral response detected in RNA-treated cultured cells of the sandfly, Lutzomyia longipalpis | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.dci.2007.06.008 | pt_BR |
| Abstract | Lutzomyia longipalpis is the principal vector of visceral leishmaniasis in the Americas, and can also transmit some viruses. To help develop a gene-silencing system for this sandfly, we transfected cultured embryonic cells with various double-stranded RNAs using West Nile virus (WNV) virus-like particles (VLPs) expressing luciferase as the target RNA to demonstrate effective gene knock-down. When luciferase dsRNA was introduced into these cells, they produced the expected reduction in VLP-encoded luciferase, suggesting specific silencing of the luciferase gene. Surprisingly, we found that unrelated dsRNAs, which included those specific for several L. longipalpis gene sequences and Escherichia coli beta-galactosidase, diminished replication of the VLP-encoded genome. These results are the first indication for a nucleic acid-induced, non-specific antiviral response in this important insect vector. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Tripanosomatídeos e Flebetomineos. Rio de Janeiro, RJ, Brasil / University of Texas Medical Branch. Department of Pathology and Sealy Center for Vaccine Development. Galveston, TX, USA. | pt_BR |
| Affilliation | University of Texas Medical Branch. Department of Pathology and Sealy Center for Vaccine Development. Galveston, TX, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Tripanosomatídeos e Flebetomineos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Lutzomyia Longipalpis | pt_BR |
| Subject | LL5 cells | pt_BR |
| Subject | Antiviral response | pt_BR |
| Subject | West Nile virus | pt_BR |
| Subject | Virus-like particle | pt_BR |
| Subject | Innate immunity | pt_BR |
| Subject | siRNA | pt_BR |
| Subject | Gene silencing | pt_BR |
| DeCS | RNA Interferente Pequeno | pt_BR |
| DeCS | Inativação Gênica | pt_BR |
| Embargo date | 2022-01-01 | |
