| Author | Blanes, Lucas | |
| Author | Saito, Renata M. | |
| Author | Genta, Fernando A. | |
| Author | Donegá, Juliana | |
| Author | Terra, Walter R. | |
| Author | Ferreira, Clélia | |
| Author | Lago, Claudimir Lucio do | |
| Access date | 2019-03-28T17:29:42Z | |
| Available date | 2019-03-28T17:29:42Z | |
| Document date | 2008 | |
| Citation | BLANES, Lucas et al. Direct detection of underivatized chitooligosaccharides produced through chitinase action using capillary zone electrophoresis. Analytical Biochemistry, v. 373, p.99-103, Oct. 2008. | pt_BR |
| ISSN | 0003-2697 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/32280 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Detecção de condutividade sem contato acoplada capacitivamente | pt_BR |
| Subject in Portuguese | Quito Oligossacarídeos | pt_BR |
| Title | Direct detection of underivatized chitooligosaccharides produced through chitinase action using capillary zone electrophoresis | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.ab.2007.08.042 | |
| Abstract | Capillary electrophoresis with capacitively coupled contactless conductivity detection was successfully used to quantify N-acetylglucosamine and five N-acetyl-chitooligosaccharides (C2-C6) produced after reaction with a purified chitinase (TmChi) from Tenebrio molitor (Coleoptera). No derivatization process was necessary. The separation was developed using 10 mM NaOH with 10% (v/v) acetonitrile as background electrolyte and homemade equipment with a system that avoids the harmful effect of electrolysis. The limit of detection for all oligosaccharides was ca. 3microM, and the results indicated that the larger the oligosaccharide, the higher the sensitivity. Analysis of the chitooligosaccharides produced revealed that TmChi has an endolytic cleavage pattern with C5 as the best substrate (higher catalytic efficiency kcat/KM) releasing C2 and C3. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Química. Departamento de Química. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Química. Departamento de Química. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Química. Departamento de Química. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Química. Departamento de Química. São Paulo, SP, Brasil. | pt_BR |
| Subject | Capillary electrophoresis | pt_BR |
| Subject | Capacitively coupled contactless conductivity detection | pt_BR |
| Subject | Chitinase | pt_BR |
| Subject | Chitooligosaccharides | pt_BR |
| DeCS | Eletroforese Capilar | pt_BR |
| DeCS | Quitinases | pt_BR |
| e-ISSN | 1096-0309 | |
| Embargo date | 2022-01-01 | |
