Author | Araújo, Lívia Caroline Alexandre de | |
Author | Purificação-Júnior, Antônio Fernando da | |
Author | Silva, Sivoneide Maria da | |
Author | Lopes, Ana Catarina Souza | |
Author | Veras, Dyana Leal | |
Author | Alves, Luiz Carlos | |
Author | Santos, Fábio Brayner dos | |
Author | Napoleão, Thiago Henrique | |
Author | Correia, Maria Tereza dos Santos | |
Author | Silva, Márcia Vanusa da | |
Author | Oliva, Maria Luiza Vilela | |
Author | Oliveira, Maria Betânia Melo de | |
Access date | 2019-03-29T12:54:25Z | |
Available date | 2019-03-29T12:54:25Z | |
Document date | 2019 | |
Citation | ARAÚJO, Lívia Caroline Alexandre de et al. In Vitro Evaluation of Mercury (Hg2+) Effects on Biofilm Formation by Clinical and Environmental Isolates of Klebsiella Pneumoniae. Ecotoxicology and Environmental Safety, v. 169, p. 669–677, Mar. 2019. | pt_BR |
ISSN | 1090-2414 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/32289 | |
Sponsorship | Coordenação de Aperfeiçoamento da Agência Brasileira de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) e FACEPE (Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco) | pt_BR |
Language | eng | pt_BR |
Rights | restricted access | pt_BR |
Title | In vitro evaluation of mercury (Hg2+) effects on biofilm formation by clinical and environmental isolates of Klebsiella pneumoniae | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.ecoenv.2018.11.036 | |
Abstract | The increase in urbanization and industrialization has contributed to the contamination of different environments by means of xenobiotic compounds, such as heavy metals, causing changes in microbial communities. Among these metals, the Mercury (Hg2+) is one the most prevalent toxic metals for the environment The present study aimed to evaluate the effect of mercury on the formation of biofilm by environmental (collected from urban stream water) and clinical isolates of Klebsiella pneumoniae. In addition, antibiotic resistance, virulence factors, and genetic diversity were investigated. Taxonomic identity of eight isolates (one reference, two clinical, and five environmental isolates) was performed by MALDI-TOF-MS, while the antibiotic susceptibility profile was assessed by the disc diffusion method. The ability to form biofilms was evaluated by culture on Congo red agar and by crystal violet staining. Biofilm structure was analyzed by scanning electron microscopy. The hydrophobicity profile and the presence of the virulence genes cps, fimH, and mrkD was investigated. The presence of merA and its relationship with antimicrobial resistance were also assessed. The identity of all isolates was confirmed by MALDI-TOF-MS, and different profiles of resistance to mercury and antibiotics as well as of biofilm formation were identified for the clinical and environmental isolates. All isolates were hydrophilic and positive for the virulence genes cps, fimH, and mrkD; only the clinical isolate K36-A2 was positive for merA. The diversity of the isolates was confirmed by ERIC-PCR, which revealed high heterogeneity among the isolates. In conclusion, the data demonstrate that the investigated isolates present different responses to exposure to Hg2+ and correspond to distinct populations of K. pneumoniae disseminated in the investigated environment. The data obtained in this work will aid in understanding the mechanisms of survival of this pathogen under adverse conditions. | pt_BR |
Affilliation | Universidade Federal de Pernambuco. Centro de Biociências. Departamento de Bioquímica. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Universidade Federal de Pernambuco. Centro de Biociências. Departamento de Bioquímica. Recife, PE, Brasil. | pt_BR |
Affilliation | Universidade Federal de Pernambuco. Centro de Ciências da Saúde. Departamento de Medicina Tropical. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Universidade Federal de Pernambuco. Centro de Biociências. Departamento de Bioquímica. Recife, PE, Brasil. | pt_BR |
Affilliation | Universidade Federal de Pernambuco. Centro de Biociências. Departamento de Bioquímica. Recife, PE, Brasil. | pt_BR |
Affilliation | Universidade Federal de Pernambuco. Centro de Biociências. Departamento de Bioquímica. Recife, PE, Brasil. | pt_BR |
Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Bioquímica. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Universidade Federal de Pernambuco. Centro de Biociências. Departamento de Bioquímica. Recife, PE, Brasil. | pt_BR |
Subject | Biofilm | pt_BR |
Subject | Co-resistance | pt_BR |
Subject | Genetic diversity | pt_BR |
Subject | Heavy metal | pt_BR |
DeCS | Antibacterianos / farmacologia | pt_BR |
DeCS | Biofilmes / efeitos de drogas | pt_BR |
DeCS | Biofilmes / crescimento e desenvolvimento | pt_BR |
DeCS | Resistência a Medicamentos, Bacteriana / efeitos de drogas | pt_BR |
DeCS | Resistência a Medicamentos Bacterianos / Genéticos | pt_BR |
DeCS | Poluentes Ambientais / toxicidade | pt_BR |
DeCS | Hospitais | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Klebsiella pneumoniae / efeitos de drogas | pt_BR |
DeCS | Klebsiella pneumoniae / genética | pt_BR |
DeCS | Klebsiella pneumoniae / patogenicidade | pt_BR |
DeCS | Mercúrio / toxicidade | pt_BR |
DeCS | Testes de Sensibilidade Microbiana | pt_BR |
DeCS | Fatores de virulência / genética | pt_BR |
Embargo date | 2050-01-01 | |