| Author | Cajado, Cyntia | |
| Author | Fukutani, Kyioshi Ferreira | |
| Author | Vilas Boas, Wendell | |
| Author | Cerqueira, Bruno Antonio Veloso | |
| Author | Tavares, Natalia Machado | |
| Author | Leite, Ivana | |
| Author | Siqueira, Isadora Cristina de | |
| Author | Lyra, Isa | |
| Author | Adorno, Elisângela Vitória | |
| Author | Goncalves, Marilda Souza | |
| Access date | 2019-04-01T14:07:51Z | |
| Available date | 2019-04-01T14:07:51Z | |
| Document date | 2013 | |
| Citation | CAJADO, Cyntia et al. Toll like receptors have mRNA differentiated expression in dendritic cells in crisis-state sickle cell anemia patients, suggesting a pivotal role of these molecules and cell type in the maintenance of inflammatory response. Blood, v. 122, p. 1-6, 2013. | pt_BR |
| ISSN | 0006-4971 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/32312 | |
| Language | eng | pt_BR |
| Publisher | American Society of Hematology | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Anemia falciforme | pt_BR |
| Subject in Portuguese | Células dendríticas | pt_BR |
| Subject in Portuguese | Inflamação | pt_BR |
| Subject in Portuguese | mRNA | pt_BR |
| Subject in Portuguese | Humanos | pt_BR |
| Title | Toll Like Receptors Have mRNA Differentiated Expression In Dendritic Cells In Crisis-State Sickle Cell Anemia Patients, Suggesting a Pivotal Role Of These Molecules and Cell Type In The Maintenance Of Inflammatory Response | pt_BR |
| Type | Article | |
| Abstract | The vascular alterations play an important role in the SCA chronic inflammatory state
with the participation of different cell types, including macrophages and dendritic cells (DCs).
Aim Here, we evaluated the mRNA expression of TLRs in DCs cells pre and post LPS stimulus and
also measured IL-1β, TNF-α and IL-10 cytokines in plasma and supernatant of DCs pre and post
LPS challenge, in order to indentify a possible role of this cell and receptor in the inflammatory
state described in sickle cell anemia.
Methods In vitro generation of immature DCs (imDC) monocyte-derived was obtained of
monocytes from peripheral blood mononuclear cells (PBMC) of seven hospitalized HbSS patients. Relative mRNA abundance of each transcript was investigated by quantitative real time PCR and cytokines levels were measured by ELISA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Instituto Nacional de Ciência e Tecnologia do Sangue. Campinas, SP, Brasil. | pt_BR |
| Affilliation | Universidade de Santa Cruz. Ilhéus, BA, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação de Hematologia e Hemoterapia da Bahia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Obras Sociais Irmã Dulce. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação de Hematologia e Hemoterapia da Bahia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Faculdade de Farmacia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, Brasil. | pt_BR |
| Subject | Anemia, Sickle Cell | pt_BR |
| Subject | Dendritic Cells | pt_BR |
| Subject | Inflammation | pt_BR |
| Subject | mRNA | pt_BR |
| Subject | Humans | pt_BR |
