Author | Corrêa, José Raimundo | |
Author | Atella, Georgia Correa | |
Author | Batista, Marcelo Meuser | |
Author | Soares, Maurilio José | |
Access date | 2019-04-04T12:53:25Z | |
Available date | 2019-04-04T12:53:25Z | |
Document date | 2008 | |
Citation | CORRÊA, José Raimundo et al. Transferrin uptake in Trypanosoma cruzi is impaired by interference on cytostome-associated cytoskeleton elements and stability of membrane cholesterol, but not by obstruction of clathrin-dependent endocytosis. Experimental Parasitology, v. 119, p. 58-66, Dec. 2008. | |
ISSN | 0014-4894 | |
URI | https://www.arca.fiocruz.br/handle/icict/32358 | |
Description | Produção científica do Laboratório de Biologia Celular. | pt_BR |
Description | Produção científica do Laboratório de Ultraestrutura Celular. | pt_BR |
Language | eng | en_US |
Publisher | Elsevier | |
Rights | restricted access | |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Subject in Portuguese | Microscopia eletrônica de transmissão | pt_BR |
Subject in Portuguese | Endocitose | pt_BR |
Subject in Portuguese | Transferrina | pt_BR |
Subject in Portuguese | Citoesqueleto | pt_BR |
Subject in Portuguese | Citostome | pt_BR |
Subject in Portuguese | Colesterol da membrana | pt_BR |
Title | Transferrin uptake in Trypanosoma cruzi is impaired by interference on cytostome-associated cytoskeleton elements and stability of membrane cholesterol, but not by obstruction of clathrin-dependent endocytosis | en_US |
Type | Article | |
DOI | 10.1016/j.exppara.2007.12.010 | |
Abstract | Transferrin uptake by Trypanosoma cruzi epimastigotes occurs mainly through the cytostome/cytopharynx. Here, we present evidences for the association of sterol-rich membrane domains with the transferrin endocytic site. Assays using pharmacological treatments to disrupt clathrin-coated pits and hinder caveolae formation showed no association between transferrin uptake and clathrin-dependent endocytosis, but indicated that cholesterol stability in membrane domains is essential for the endocytosis of transferrin. Furthermore, it was observed a connection between the integrity of cytoskeleton elements at the cytopharynx and the function of the cytostome. Our data show that T. cruzi epimastigotes depend on a specialized pathway for transferrin uptake, which is cholesterol-dependent, clathrin-independent, and closely associated with the structural stability of the cytostome/cytopharynx cytoskeleton. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil. | |
Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil. | |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | |
Affilliation | Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | |
Subject | Trypanosoma cruzi | en_US |
Subject | Transmission electron microscopy | en_US |
Subject | Transferrin | en_US |
Subject | Endocytosis | en_US |
Subject | Cytoskeleton | en_US |
Subject | Membrane cholesterol | en_US |
Subject | Clathrin | en_US |
e-ISSN | 1090-2449 | |