| Author | Santos, Débora Regina Lopes dos | |
| Author | Villar, Livia Melo | |
| Author | Paula, Vanessa Salete de | |
| Author | Lima, Gerson Silva de | |
| Author | Gaspar, Ana Maria Coimbra | |
| Access date | 2019-04-04T15:58:58Z | |
| Available date | 2019-04-04T15:58:58Z | |
| Document date | 2008 | |
| Citation | SANTOS, Débora Regina Lopes dos et al. Hepatitis A virus subgenotypes dissemination during a community outbreak in a surrounding region of Rio de Janeiro. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 3, p. 254-258, May 2008. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/32374 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Vírus da Hepatite A | pt_BR |
| Subject in Portuguese | Epidemiologia molecular | pt_BR |
| Subject in Portuguese | Co-circulação dos subgenótipos IA e IB | pt_BR |
| Title | Hepatitis A virus subgenotypes dissemination during a community outbreak in a surrounding region of Rio de Janeiro | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1590/S0074-02762008000300005 | pt_BR |
| Abstract | From December 1999 to December 2001, many cases of hepatitis A were notified in the county of Belford Roxo involving individuals aged 0 to 79 years. Serum samples were collected to evaluate the prevalence of anti-hepatitis A virus (HAV) antibodies, to detect HAV-RNA and to correlate with possible risk factors of HAV infection. Serum samples were screened by commercial IgM and total anti-HAV antibody ELISA and HAV-RNA was isolated and subsequently amplified by reverse transcription-polymerase chain reaction (RT-PCR) at VP1/2A region, sequenced and analyzed. Total anti-HAV prevalence was 87.9% (203/231) and IgM anti-HAV prevalence was 38.7% (89/231). Multivariate analysis showed that individuals under 20 years old are risks groups to acquire the infection suggesting that hygienic habits of young subjects are the principal factor of transmission and so they could be the target for vaccine programs. HAV-RNA was amplified from 29 (32.5%) IgM anti-HAV positive patients and 26 samples were sequenced and classified into subgenotypes IB (8 isolates) and IA (18 isolates). Isolates classified into subgenotype IB were identical representing one distinct strain. We could observe both subgenotypes circulating during the study which suggests different sources of infection. Prophylactic measures as vaccination strategies added to improvements in hygienic and sanitary conditions would be highly effective to reduction of infection. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Departamento de Reativos para Diagnóstico Setor de Bactérias e Recombinantes. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Hepatitis A virus | pt_BR |
| Subject | Molecular epidemiology | pt_BR |
| Subject | Co-circulation of subgenotypes IA and IB | pt_BR |
| e-ISSN | 1678-8060 | |
