| Author | Plotkowski, Maria Cristina | |
| Author | Brandão, Bruno A. | |
| Author | Assis, Maria Cristina de | |
| Author | Feliciano, Luis Felipe P. | |
| Author | Raymond, Benoit | |
| Author | Freitas, Carla | |
| Author | Saliba, Alessandra M. | |
| Author | Zahm, Jean Marie | |
| Author | Touqui, Lhousseine | |
| Author | Bozza, Patrícia T. | |
| Access date | 2019-04-16T12:25:48Z | |
| Available date | 2019-04-16T12:25:48Z | |
| Document date | 2008 | |
| Citation | PLOTKOWSKI, Maria Cristina et al. Lipid body mobilization in the ExoU-induced release of inflammatory mediators by airway epithelial cells. Microbial Pathogenesis, v. 45, p. 30-37, 2008. | pt_BR |
| ISSN | 0882-4010 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/32508 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Pseudomonas aeruginosa | pt_BR |
| Subject in Portuguese | Resposta inflamatória | pt_BR |
| Subject in Portuguese | Corpo lipídico | pt_BR |
| Subject in Portuguese | Ácido araquidônico | pt_BR |
| Title | Lipid body mobilization in the ExoU-induced release of inflammatory mediators by airway epithelial cells | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.micpath.2008.01.008 | |
| Abstract | This report addressed the question whether ExoU stimulation of airway epithelial cells may contribute to
the inflammatory response detected in the course of Pseudomonas aeruginosa respiratory infections.
Infection with PA103 P. aeruginosa elicited a potent release of IL-6 and IL-8, as well as of arachidonic acid
(AA) and PGE2 that was reduced by the bacterial treatment with MAFP, a cPLA2 inhibitor. Airway cells
from the BEAS-2B line and in primary culture were shown to be enriched in lipid bodies (LBs), that are
cytoplasmic domains implicated in AA transformation into eicosanoids. However, cells infected with
PA103 and with a mutant deficient in exoU but complemented with a functional gene exhibited reduced
contents of LBs, and this reduction was inhibited by MAFP. FACS analysis showed that the decrease in the
LB content correlated with the presence of intracellular PGE2. Also, in PA103-infected cells, PGE2 was
immunolocalized in LBs, suggesting that the reduction in the cell content of the organelles was due to
consumption of their glycerolipids, resulting in local synthesis of the prostanoid. In conclusion, we
showed the ExoU ability to induce airway epithelial cells to overproduce PGE2 and we speculate that LB
may represent intracellular loci involved in ExoU-induced eicosanoid synthesis. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Institut Pasteur. Unité de Defense Innée et Inflammation. INSERM U 874. Paris, France. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Université de Reims Champagne-Ardenne. INSERM U 903. Reims, France. | pt_BR |
| Affilliation | Institut Pasteur. Unité de Defense Innée et Inflammation. INSERM U 874. Paris, France. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Pseudomonas aeruginosa | pt_BR |
| Subject | ExoU | pt_BR |
| Subject | Lipid body | pt_BR |
| Subject | Inflammatory response | pt_BR |
| Subject | Arachidonic acid | pt_BR |
| Subject | PGE2 | pt_BR |
| DeCS | Dinoprostona | pt_BR |
| e-ISSN | 1096-1208 | |
| Embargo date | 2022-01-01 | |
