| Author | Oliveira Junior, Francisco Odencio Rodrigues de | |
| Author | Alves, Carlos Roberto | |
| Author | Calvet, Cláudia Magalhães | |
| Author | Toma, Leny | |
| Author | Bouças, Rodrigo Ippolito | |
| Author | Nader, Helena Bociani | |
| Author | Côrtes, Luzia Monteiro de Castro | |
| Author | Krieger, Marco Aurélio | |
| Author | Meirelles, Maria de Nazareth Silveira Leal de | |
| Author | Pereira, Mirian Claudia de Souza | |
| Access date | 2019-04-16T13:32:17Z | |
| Available date | 2019-04-16T13:32:17Z | |
| Document date | 2008 | |
| Citation | OLIVEIRA JUNIOR, Francisco Odencio Rodrigues de et al. Trypanosoma cruzi heparin-binding proteins and the nature of the host cell heparan sulfate-binding domain. Microbial Pathogenesis, v. 44, n. 4, p. 329-338, Apr. 2008. | |
| ISSN | 0882-4010 | |
| URI | https://www.arca.fiocruz.br/handle/icict/32513 | |
| Description | Produção científica do Laboratório de Ultraestrutura Celular. | pt_BR |
| Description | Produção científica do Laboratório de Biologia Molecular e Doenças Endêmicas. | pt_BR |
| Language | eng | en_US |
| Publisher | Elsevier | |
| Rights | restricted access | |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Cardiomiócitos | pt_BR |
| Subject in Portuguese | Glicosaminoglicanos | pt_BR |
| Subject in Portuguese | Processo de reconhecimento | pt_BR |
| Subject in Portuguese | Cromatografia | pt_BR |
| Title | Trypanosoma cruzi heparin-binding proteins and the nature of the host cell heparan sulfate-binding domain | en_US |
| Type | Article | |
| DOI | 10.1016/j.micpath.2007.10.003 | |
| Abstract | Trypanosoma cruzi invasion is mediated by receptor-ligand recognition between the surfaces of both parasite and target cell. We have previously demonstrated the role of heparan sulfate proteoglycan in the attachment and invasion of T. cruzi in cardiomyocytes. Herein, we have isolated the T. cruzi heparin-binding proteins (HBP-Tc) and investigated the nature of cardiomyocyte heparan sulfate (HS)-binding site to the parasite surface ligand. Two major heparin-binding proteins with molecular masses of 65.8 and 59 kDa were observed in total extract of amastigote and trypomastigote forms of T. cruzi. Hydrophobic [S(35)]methionine labeled proteins eluted from heparin-sepharose affinity chromatography also revealed both proteins in trypomastigotes but only the 59 kDa is strongly recognized by biotin-conjugated glycosaminoglycans. Competition assays were performed to analyze the role of sulfated proteoglycans, including heparin, keratan sulfate and both acetylated and highly sulfated domains of heparan sulfate, in the recognition and invasion process of T. cruzi. Significant inhibitions of 84% and 35% in the percentage of infection were revealed after treatment of the parasites with heparin and the N-acetylated/ N-sulfated heparan sulfate domain, respectively, suggesting the important role of the glycuronic acid and NS glucosamine domain of the HS chain in the recognition of the HBP-Tc during the T. cruzi-cardiomyocyte interaction. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Bioquímica. São Paulo, SP, Brasil. | |
| Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Bioquímica. São Paulo, SP, Brasil. | |
| Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Bioquímica. São Paulo, SP, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil. | |
| Subject | Trypanosoma cruzi | en_US |
| Subject | Cardiomyocytes | en_US |
| Subject | Glycosaminoglycans | en_US |
| Subject | Recognition process | en_US |
| Subject | Chromatography | en_US |
| e-ISSN | 1096-1208 | |
