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Autor | Chaves, J. Q. | |
Autor | Cavados, C. F. G. | |
Autor | Rabinovitch, L. | |
Fecha de acceso | 2019-04-16T17:47:10Z | |
Fecha de disponibilización | 2019-04-16T17:47:10Z | |
Fecha de publicación | 2008 | |
Referencia | CHAVES, J. Q.; CAVADOS, C. F. G.; RABINOVICH, L. Phenotypic and genotypic features of new autoagglutinating Bacillus thuringiensis strains. Journal of Invertebrate Pathology, v. 98, p. 85-92, 2008. | pt_BR |
ISSN | 0022-2011 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/32535 | |
Idioma | eng | pt_BR |
Editor | Elsevier | pt_BR |
Derechos de autor | restricted access | |
Palabras clave en Portugués | Bacillus thuringiensis | pt_BR |
Palabras clave en Portugués | Aedes aegypti | pt_BR |
Palabras clave en Portugués | Culex quinquefasciatus | pt_BR |
Palabras clave en Portugués | Aedes aegypti | pt_BR |
Palabras clave en Portugués | Cepas de mosquitos | pt_BR |
Título | Phenotypic and genotypic features of new autoagglutinating Bacillus thuringiensis strains | pt_BR |
Tipo del documento | Article | |
DOI | 10.1016/j.jip.2008.01.002 | |
Resumen en Inglés | A total of 28 autoagglutinating strains of Bacillus thuringiensis were isolated from different ecologic niches and distinct sites. Twenty-six strains demonstrated toxicity to mosquito larvae of Aedes aegypti and Culex quinquefasciatus. The electrophoretic protein profiles of the crystal components were studied. Twenty-three out of the 28 strains showed the same larvicidal activity and the same protein profiles as B. thuringiensis serovar israelensis. Using isoenzyme analysis (MLEE), it was observed the presence of three electrophoretic types (ETs). The mosquitocidal strains grouped into one ET. The random amplified polymorphic DNA analysis (RAPD) was evaluated using six primers, which demonstrated three different patterns for the 28 autoagglutinating strains, allowing correlation of the profiles obtained with the toxicity observed in the bioassays. The RAPD patterns for mosquitocidal strains were identical to the one of serovar israelensis. However, to strains of low toxicity, each primer generated distinctive RAPD patterns, which demonstrated that these strains belong to different serovars. Although the antigenic classification the 26 autoagglutinating strains of B. thuringiensis could not be determined by classical flagellar serotyping, MLEE and RAPD profiles proved these strains to be compatible with B. thuringiensis serovar israelensis. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ. Brasil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ. Brasil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ. Brasil. | pt_BR |
Palavras clave en Inglês | Bacillus thuringiensis | pt_BR |
Palavras clave en Inglês | Autoagglutinating strains | pt_BR |
Palavras clave en Inglês | Aedes aegypti | pt_BR |
Palavras clave en Inglês | Culex quinquefasciatus | pt_BR |
Palavras clave en Inglês | Mosquitocidal strains | pt_BR |
Palavras clave en Inglês | MLEE | pt_BR |
Palavras clave en Inglês | SDS-PAGE | pt_BR |
Palavras clave en Inglês | RAPR-PCR | pt_BR |
DeCS | Eletroforese em Gel de Poliacrilamida | pt_BR |
DeCS | Técnica de Amplificação ao Acaso de DNA Polimórfico | pt_BR |
e-ISSN | 1096-0805 | |
Fecha de embargo | 2022-01-01 |
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