| Author | Guimarães, Allan Jefferson | |
| Author | Hamilton, Andrew John | |
| Author | Guedes, Herbert Leonel de M. | |
| Author | Nosanchuk, Joshua Daniel | |
| Author | Zancopé-Oliveira, Rosely Maria | |
| Access date | 2019-04-18T15:32:19Z | |
| Available date | 2019-04-18T15:32:19Z | |
| Document date | 2008 | |
| Citation | GUIMARÃES, Allan Jefferson et al. Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum. Plos One, v. 3, n. 10, p. 1-17, Oct. 2008. | pt_BR |
| ISSN | 1932-6203 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/32603 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Histoplasma capsulatum | pt_BR |
| Subject in Portuguese | Antígeno M | pt_BR |
| Subject in Portuguese | Função Biológica | pt_BR |
| Subject in Portuguese | Mapeamento Molecular | pt_BR |
| Subject in Portuguese | Fase de Levedura | pt_BR |
| Title | Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.pone.0003449 | |
| Abstract | Histoplasmosis, due to the intracellular fungus Histoplasma capsulatum, can be diagnosed by demonstrating the presence of antibodies specific to the immunodominant M antigen. However, the role of this protein in the pathogenesis of histoplasmosis has not been elucidated. We sought to structurally and immunologically characterize the protein, determine yeast cell surface expression, and confirm catalase activity. A 3D-rendering of the M antigen by homology modeling revealed that the structures and domains closely resemble characterized fungal catalases. We generated monoclonal antibodies (mAbs) to the protein and determined that the M antigen is present on the yeast cell surface and in cell wall/cell membrane preparations. Similarly, we found that the majority of catalase activity was in extracts containing fungal surface antigens and that the M antigen is not significantly secreted by live yeast cells. The mAbs also identified unique epitopes on the M antigen. The localization of the M antigen to the cell surface of H. capsulatum yeast and the characterization of the protein’s major epitopes have important implications since it demonstrates that although the protein may participate in protecting the fungus against oxidative stress it is also accessible to host immune cells and antibody. | pt_BR |
| Affilliation | Albert Einstein College of Medicine of Yeshiva University. Department of Medicine and Microbiology and Immunology. Division of Infectious Diseases. Bronx, NY, USA / Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | St John’s Institute of Dermatology. Guy’s Hospital. King’s College, London, United Kingdom. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Albert Einstein College of Medicine of Yeshiva University. Department of Medicine and Microbiology and Immunology. Division of Infectious Diseases. Bronx, NY, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Histoplasma capsulatum | pt_BR |
| Subject | M antigen | pt_BR |
| Subject | Biological Function | pt_BR |
| Subject | Molecular Mapping | pt_BR |
| Subject | Yeast Phase | pt_BR |
