| Author | Amado, Luciane A. | |
| Author | Villar, Livia M. | |
| Author | Paula, Vanessa Salete de | |
| Author | Gaspar, Ana Maria C. | |
| Access date | 2019-04-18T16:08:47Z | |
| Available date | 2019-04-18T16:08:47Z | |
| Document date | 2008 | |
| Citation | AMADO, Luciane A. et al. Comparison between serum and saliva for the detection of hepatitis A virus RNA. Journal of Virological Methods, v. 148, p. 74–80, 2008. | pt_BR |
| ISSN | 0166-0934 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/32607 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Hepatite A | pt_BR |
| Subject in Portuguese | Reação em Cadeia da Polimerase em Tempo Real | pt_BR |
| Subject in Portuguese | Fluido oral | pt_BR |
| Title | Comparison between serum and saliva for the detection of hepatitis A virus RNA | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.jviromet.2007.10.020 | |
| Abstract | Due to the ease of collection, oral fluid is being investigated as an alternative to serum for diagnostic and epidemiological purposes. However,
for prospective studies involving hepatitis A virus (HAV) RNA detection, a standard methodology must be developed. In the present study, nested
RT-PCR and real-time PCR were optimized and evaluated for HAV detection and quantification, using oral fluid from healthy volunteers (n = 20)
and paired serum/oral fluid samples from individuals involved in a hepatitis A outbreak (n = 78). Using nested RT-PCR, HAV RNA was detected
in 50% of oral fluid and in 42% of serum samples from acute cases, as well as in 12% of all samples from cases without IgM and total anti-HAV.
Using real-time PCR, HAV RNA was detected in 61% of oral fluid and in 71% of serum samples from acute cases, as well as in 17 and 12%,
respectively, from patients without HAV markers. Mean viral loads were 1.7±3.24×103 copies/ml in oral fluid and 2.8±6.46×103 copies/ml
in serum. Although nested RT-PCR and real-time PCR both detected HAV RNA in oral fluid, real-time PCR was more sensitive. Oral fluid sample
testing could be used as a noninvasive method of detecting HAV RNA during HAV outbreaks. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Oral fluid | pt_BR |
| Subject | Hepatitis A | pt_BR |
| Subject | Nested RT-PCR | pt_BR |
| Subject | Real-time PCR | pt_BR |
| e-ISSN | 1879-0984 | |
| Embargo date | 2022-01-01 | |
