| Author | Brelaz-de-Castro, Maria Carolina Accioly | |
| Author | Almeida, Thays Miranda de | |
| Author | Almeida, Amanda Ferreira de | |
| Author | Souza, Marina de Assis | |
| Author | Mendes, Andresa Pereira de Oliveira | |
| Author | Silva, Rafael de Freitas E. | |
| Author | Brito, Maria Edileuza Felinto de | |
| Author | Pereira, Valéria Rêgo Alves | |
| Access date | 2019-04-22T12:30:34Z | |
| Available date | 2019-04-22T12:30:34Z | |
| Document date | 2018 | |
| Citation | BRELAZ-DE-CASTRO, M. C. A. et al. Combination of Flow Cytometry and QPCR to Study the Immune Response of American Cutaneous Leishmaniasis Patients. Microbial Pathogenesis, v. 123, p. 433–439, out. 2018. | pt_BR |
| ISSN | 1096-1208 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/32648 | |
| Sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | pt_BR |
| Language | eng | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Combination of flow cytometry and qPCR to study the immune response of american cutaneous leishmaniasis patients | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.micpath.2018.07.044 | |
| Abstract | American Cutaneous leishmaniasis (ACL) is a public health problem. The immunological response is mainly dependent on T cell cytokine responses and might influence disease presentation, susceptibility and development. The understanding of the host immune response role in the control and in the pathology of leishmaniasis is relevant and has implications on diagnosis, follow-up and vaccine development. In this study, the differences in the immune response and T cell profile of patients before treatment was investigated through flow cytometry and real time PCR in peripheral blood mononuclear cells after different antigenic stimulations. Among the main findings are the significant presence of TNF and IFN-γ gene expression after 24 h of in vitro stimulation, and 48 h later the presence of CD4+ T and CD8+ T cells producing IL-10 and IL-4. This may be due to the differences in cytokine release over time and the presence of cells other than lymphocytes influencing the mRNA transcript detection. Evaluation of the immune response of individuals with leishmaniasis or other diseases should associate different technologies and times points for a clear and more reliable assessment of the immune response. This would help in the design of vaccine strategies/immunotherapies. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / Universidade Federal de Pernambuco. Núcleo de Enfermagem.Vitória de Santo Antão, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / Universidade de Pernambuco.Departamento de Ciências Naturais e Exatas. Garanhuns, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Subject | American cutaneous leishmaniasis | pt_BR |
| Subject | Cytokines | pt_BR |
| Subject | Flow cytometry | pt_BR |
| Subject | Immune response | pt_BR |
| Subject | Leishmania braziliensis | pt_BR |
| Subject | T lymphocytes | pt_BR |
| Subject | qPCR | pt_BR |
| DeCS | Adolescente | pt_BR |
| DeCS | Adulto | pt_BR |
| DeCS | Antígenos, Protozoários / imunologia | pt_BR |
| DeCS | Brasil | pt_BR |
| DeCS | Linfócitos T CD4-Positivos / metabolismo | pt_BR |
| DeCS | Linfócitos T CD8-Positivos / metabolismo | pt_BR |
| DeCS | Técnicas de Cultura Celular | pt_BR |
| DeCS | Citocinas / metabolismo | pt_BR |
| DeCS | Citometria de Fluxo / métodos | pt_BR |
| DeCS | Humanos | pt_BR |
| DeCS | Interferon-gama / genética | pt_BR |
| DeCS | Interferon-gamma / metabolismo | pt_BR |
| DeCS | Interleucina-10 / metabolismo | pt_BR |
| DeCS | Interleucina-4 / metabolismo | pt_BR |
| DeCS | Leishmania braziliensis / imunologia | pt_BR |
| DeCS | Leishmaniose cutânea / sangue | pt_BR |
| DeCS | Leishmaniose cutânea / imunologia | pt_BR |
| DeCS | Leucócitos Mononucleares / metabolismo | pt_BR |
| DeCS | Meia idade | pt_BR |
| DeCS | Proteínas Protozoárias / imunologia | pt_BR |
| DeCS | RNA mensageiro / metabolismo | pt_BR |
| DeCS | Reação em Cadeia da Polimerase em Tempo Real / métodos | pt_BR |
| DeCS | Linfócitos T | pt_BR |
| DeCS | Adulto jovem | pt_BR |
| Embargo date | 2050-01-01 | |
