| Author | Bezerra Neto, Armando Monteiro | |
| Author | Rabelo, Marcelle Aquino | |
| Author | Lima, Jailton Lobo da Costa | |
| Author | Loibman, Stéfany Ojaimi | |
| Author | Leal, Nilma Cintra | |
| Author | Maciel, Maria Amélia Vieira | |
| Access date | 2019-04-22T13:45:05Z | |
| Available date | 2019-04-22T13:45:05Z | |
| Document date | 2018 | |
| Citation | BEZERRA NETO, A. M. et al. Occurrence of the VanA Gene in Staphylococcus Epidermidis from Nasopharyngeal Secretion of Health-Care Workers, Recife, Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 51, n. 3, p. 304-309, jun. 2018. | pt_BR |
| ISSN | 1678-9849 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/32658 | |
| Sponsorship | Fundação de Amparo Ciência do
Estado de Pernambuco (FACEPE) to MAVM (Process APQ 0579-2.12/06)
and the Conselho Nacional Científico e Tecnológico (CNPq) Grant to MAVM
(Process #474131/2011-4). | pt_BR |
| Language | eng | pt_BR |
| Rights | open access | pt_BR |
| Title | Occurrence of the vanA gene in Staphylococcus epidermidis from nasopharyngeal secretion of Health-Care Workers, Recife, Brazil | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1590/0037-8682-0159-2017 | |
| Abstract | Introduction: The increasing reports of vancomycin-resistant Staphylococcus strains (VRS) haves caused concern worldwide,
from the laboratory detection to patient management. This study aimed to identify the occurrence of VRS strains among healthcare
professionals from a university hospital. Methods: A total of 102 Staphylococcus sp. isolates from healthcare professionals,
obtained in a previous study were evaluated according to standard techniques for VRS detection. Results: After screening
inoculation of plates containing 6µg/ml of vancomycin, 19 resistant isolates were identified. The susceptibility profile to other
antimicrobials revealed 18 multidrug resistant isolates. The minimum inhibitory concentration (MIC) was determined by E-test
and broth microdilution. According to E-tests, of 19 isolates grown in BHI-V6, four isolates presented MIC ≥ 128 µg/ml, seven
with MIC ranging from 4 to 8 µg/ml, and eight with MIC ≤ 2µg/ml. By broth microdilution, 14 isolates presented MIC ≤ 2 µg/
ml and five with MIC ≥ 16µg/ml. The presence of the gene vanA was determined by PCR in the five resistant isolates, and this
gene was detected in one of the strains. Furthermore, among the 19 strains, the gene mecA was found in 13 (39,4%) isolates,
including the strain carrying the gene vanA. Conclusions: Based on these results, we highlight the presence of one strain carrying
both vanA and the mecA genes, as well as multidrug-resistant strains colonizing healthcare professionals, and their importance as
potential vectors to spread strains carrying resistance genes in the hospital environment. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Departamento de Medicina Tropical. Programa de Pós-Graduação em Medicina Tropical. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Departamento de Medicina Tropical. Programa de Pós-Graduação em Medicina Tropical. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Departamento de Medicina Tropical. Programa de Pós-Graduação em Medicina Tropical. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Centro de Biociências. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Departamento de Medicina Tropical. Programa de Pós-Graduação em Medicina Tropical. Recife, PE, Brasil / Universidade Federal de Pernambuco. Centro de Ciências da Saúde. Departamento de Microbiologia. Recife, PE, Brasil. | pt_BR |
| Subject | Staphylococcus | pt_BR |
| Subject | Healthcare professionals | pt_BR |
| Subject | Vancomycin | pt_BR |
| Subject | Multiresistance | pt_BR |
| Subject | Methicillin | pt_BR |
| DeCS | Antibacterianos / farmacologia | pt_BR |
| DeCS | Proteínas Bacterianas / genética | pt_BR |
| DeCS | Ligases de carbono-oxigênio / genética | pt_BR |
| DeCS | Pessoal de Saúde | pt_BR |
| DeCS | Humanos | pt_BR |
| DeCS | Resistência à Meticilina / genética | pt_BR |
| DeCS | Testes de Sensibilidade Microbiana | pt_BR |
| DeCS | Nasofaringe / microbiologia | pt_BR |
| DeCS | Reação em Cadeia da Polimerase | pt_BR |
| DeCS | Staphylococcus epidermidis / efeitos de drogas | pt_BR |
| DeCS | Staphylococcus epidermidis / genetics | pt_BR |
| DeCS | Staphylococcus epidermidis / isolamento & purificação | pt_BR |
| DeCS | Resistência à vancomicina / genética | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
