| Author | Souza, Elen Mello de | |
| Author | Batista, Marcelo Meuser | |
| Author | Batista, Denise da Gama Jaén | |
| Author | Duarte, Bianca Borges | |
| Author | Araujo-Jorge, Tania Cremonini de | |
| Author | Soeiro, Maria de Nazaré Correia | |
| Access date | 2019-04-25T16:51:56Z | |
| Available date | 2019-04-25T16:51:56Z | |
| Document date | 2008 | |
| Citation | SOUZA, Elen Mello de et al. Trypanosoma cruzi: alpha-2-macroglobulin regulates host cell apoptosis induced by the parasite infection in vitro. Experimental Parasitology, v. 118, n. 3, p. 331-337, Sept. 2008. | |
| ISSN | 0014-4894 | |
| URI | https://www.arca.fiocruz.br/handle/icict/32752 | |
| Description | Produção científica do Laboratório de Biologia Celular. | pt_BR |
| Language | eng | en_US |
| Publisher | Elsevier | |
| Rights | restricted access | |
| Subject in Portuguese | DNA | pt_BR |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Tipo I morte celular programada | pt_BR |
| Subject in Portuguese | Apoptose de células hospedeiras | pt_BR |
| Subject in Portuguese | Ácido desoxirribonucleico | pt_BR |
| Title | Trypanosoma cruzi: alpha-2-macroglobulin regulates host cell apoptosis induced by the parasite infection in vitro | en_US |
| Type | Article | |
| DOI | 10.1016/j.exppara.2007.09.004 | |
| Abstract | A2M is a broad spectrum proteinase inhibitor and cytokine carrier, besides presenting anti-apoptotic activity through the binding to its receptor, LRP. During Trypanosoma cruzi infection, apoptosis of host cells and intracellular parasites is commonly observed both in vivo and in vitro. Since plasma as well as tissue A2M levels are increased in both murine and human acute T. cruzi infection, we evaluated the possible role of A2M (its methylamine transformed Fast form—A2M-F) in regulating apoptotic events in peritoneal macrophages and cardiomyocytes during in vitro interaction with the parasite. Our data showed that DNA fragmentation (a hallmark of apoptosis) of both host cells and parasites was inhibited by A2M-F. Impaired apoptosis was also noted when A2M-F was added to the cultures maintained under serum deprivation. In addition, macrophages from C57/BL6 mice, known to display higher LRP levels as compared to those of C3H lineage, displayed higher reduction in the apoptotic levels during the A2M-F treatment. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | |
| Subject | A2M | en_US |
| Subject | alpha-2-macroglobulin | en_US |
| Subject | Type I-PCD | en_US |
| Subject | type I programmed cell death | en_US |
| Subject | TUNEL | en_US |
| Subject | TdT-mediated dUTP nick endlabeling | en_US |
| Subject | DNA | en_US |
| Subject | Deoxyribonucleic acid | en_US |
| Subject | Trypanosoma cruzi | en_US |
| Subject | Serum deprivation | en_US |
| DeCS | Marcação In Situ das Extremidades Cortadas | pt_BR |
| e-ISSN | 1090-2449 | |
