| Author | Santos, Dayane Kelly Dias do Nascimento | |
| Author | Almeida, Vanessa Silva de | |
| Author | Araujo, Daniel Rodrigo Cavalcante de | |
| Author | Harand, Wolfgang | |
| Author | Soares, Ana Karine de Araújo | |
| Author | Moreira, Leyllane Rafael | |
| Author | Lorena, Virgínia Maria Barros de | |
| Author | Magalhães, Lucimeri Paulino Machado | |
| Author | Ximenes, Rafael Matos | |
| Author | Sena, Kêsia Xisto da Fonseca Ribeiro de | |
| Author | Melo, Cristiane Moutinho Lagos de | |
| Author | Napoleão, Thiago Henrique | |
| Author | Lima, Cláudia Sampaio de Andrade | |
| Author | Yara, Ricardo | |
| Author | Vieira, Jeymesson Raphael Cardoso | |
| Access date | 2019-04-29T13:56:25Z | |
| Available date | 2019-04-29T13:56:25Z | |
| Document date | 2018 | |
| Citation | SANTOS, D. K. D. do N. et al. Evaluation of Cytotoxic, Immunomodulatory and Antibacterial Activities of Aqueous Extract from Leaves of Conocarpus Erectus Linnaeus (Combretaceae). Journal of Pharmacy and Pharmacology, v. 70, n. 8, p. 1092–1101, 2018. | pt_BR |
| ISSN | 2042-7158 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/32826 | |
| Sponsorship | CAPES (Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior) | pt_BR |
| Language | eng | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Evaluation of cytotoxic, immunomodulatory and antibacterial activities of aqueous extract from leaves of Conocarpus erectus Linnaeus (Combretaceae) | pt_BR |
| Type | Article | |
| DOI | 10.1111/jphp.12930 | |
| Abstract | OBJECTIVES: This work evaluated the antibacterial activity, cytotoxicity and immunomodulatory effect on human peripheral blood mononuclear cells (PBMCs) promoted by aqueous extract from Conocarpus erectus leaves (AELCe). METHODS: The extract was characterized by thin layer chromatography and ultraperformance liquid chromatography coupled to mass spectrometry (UPLC-MS). Cytotoxicity of AELCe (6.25-50 μg/ml) was investigated using annexin V and propidium iodide. Cytokine and nitric oxide levels in PBMCs culture supernatants exposed or not to AELCe (12.5 μg/ml) were determined, and antibacterial activity was evaluated by disc diffusion and broth microdilution methods. KEY FINDINGS: AELCe contained 3',4'-OH flavonoids, phenylpropanoglycosides, saponins, polymeric proanthocyanidins and hydrolysable tannins. Moreover, 10 other compounds were identified through UPLC-MS technique. AELCe did not affect lymphocyte viability at 6.25 and 12.5 μg/ml. IL-2, IL-10, TNF-α, IFN-γ and nitric oxide was produced in higher levels by cells treated with AELCe. Proliferation and activation of CD8+ T lymphocytes were also stimulated. AELCe showed bacteriostatic activity against clinical and antibiotic-resistant strains of Staphylococcus aureus (MIC between 250 and 1000 μg/ml). CONCLUSIONS: AELCe showed a moderate bacteriostatic activity and promoted an immunomodulatory status through higher production of Th1 cytokines, nitric oxide release and T CD8+ lymphocytes stimulation. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Departamento de Histologia e Embriologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Departamento de Histologia e Embriologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Instituto Nacional do Semiárido (INSA). Campina Grande, Brasil. | pt_BR |
| Affilliation | Instituto Nacional do Semiárido (INSA). Campina Grande, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Departamento de Antibióticos. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Departamento de Antibióticos. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Departamento de Antibióticos. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Departamento de Antibióticos. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Departamento de Bioquímica. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Departamento de Biofísica e Radiobiologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Departamento de Engenharia Biomédica. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Departamento de Histologia e Embriologia. Recife, PE, Brasil. | pt_BR |
| Subject | Conocarpus erectus | pt_BR |
| Subject | Lymphocyte activation | pt_BR |
| Subject | Mangrove | pt_BR |
| Subject | Staphylococcus aureus | pt_BR |
| Subject | Th1response | pt_BR |
| DeCS | Adulto | pt_BR |
| DeCS | Antibacterianos / isolamento e purificação | pt_BR |
| DeCS | Antibacterianos / farmacologia | pt_BR |
| DeCS | Antibacterianos / toxicidade | pt_BR |
| DeCS | Sobrevivência Celular / efeitos de drogas | pt_BR |
| DeCS | Células Cultivadas | pt_BR |
| DeCS | Combretaceae / química | pt_BR |
| DeCS | Citocinas / análise | pt_BR |
| DeCS | Humanos | pt_BR |
| DeCS | Fatores Imunológicos / isolamento e purificação | pt_BR |
| DeCS | Fatores Imunológicos / farmacologia | pt_BR |
| DeCS | Leucócitos Mononucleares / efeitos de drogas | pt_BR |
| DeCS | Leucócitos Mononucleares / imunologia | pt_BR |
| DeCS | Linfócitos / efeitos de drogas | pt_BR |
| DeCS | Linfócitos / imunologia | pt_BR |
| DeCS | Testes de Sensibilidade Microbiana | pt_BR |
| DeCS | Extratos Vegetais / isolamento e purificação | pt_BR |
| DeCS | Extratos Vegetais / Farmacologia | pt_BR |
| DeCS | Extratos vegetais / toxicidade | pt_BR |
| DeCS | Folhas de plantas / química | pt_BR |
| DeCS | Pseudomonas aeruginosa / efeitos de drogas | pt_BR |
| DeCS | Staphylococcus aureus / efeitos de drogas | pt_BR |
| DeCS | Adulto jovem | pt_BR |
| Embargo date | 2050-01-01 | |
