| Author | Santos, Beatriz Senra Álvares da Silva | |
| Author | Pessoa, Natalia Lima | |
| Author | Lucinda, Natalia | |
| Author | Oliveira, Gustavo Cardoso de | |
| Author | Silva, Thais Souza | |
| Author | Andrade, Ketyllen Reis | |
| Author | Galvão Filho, Bruno | |
| Author | Rocha, Marcele Neves | |
| Author | Machado, Alexandre Vieira | |
| Author | Alves, Pedro Augusto | |
| Author | Alves, Érica Alessandra Rocha | |
| Author | Kroon, Erna Geessien | |
| Author | Campos, Marco Antônio | |
| Access date | 2019-04-30T12:17:27Z | |
| Available date | 2019-04-30T12:17:27Z | |
| Document date | 2018 | |
| Citation | SANTOS, Beatriz Senra Álvares da Silva et al. Subcutaneous Model for the Study of Dengue Virus Infection in Immune Competent Mice. Journal of Biosciences and Medicines, v. 06, n. 05, p. 97-110, 2018. | pt_BR |
| ISSN | 2327-5081 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/32867 | |
| Language | eng | pt_BR |
| Publisher | Scientific Research Publishing | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Modelo de Murino Subcutâneo | pt_BR |
| Subject in Portuguese | Doença da Dengue | pt_BR |
| Subject in Portuguese | Rato imune competente | pt_BR |
| Subject in Portuguese | Citocinas | pt_BR |
| Subject in Portuguese | Resposta imune inata | pt_BR |
| Title | Subcutaneous Model for the Study of Dengue Virus Infection in Immune Competent Mice | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.4236/jbm.2018.65011 | |
| Abstract | Various mouse models to study dengue have been described by different au-thors, some of them using immunodeficient or some using humanized mice. Our group reported previously a deadly murine model, which used the intra-cranial inoculum of highly virulent Dengue virus (DENV) in immune com-petent mouse. Here we present a model of immune competent mouse (C57BL/6), infected subcutaneously by the same highly virulent DENV (DENV3 genotype I). In this immunocompetent systemic mice model, the cytokine levels and hematological parameters such as total and differential leukocyte and platelets counts, together with weight loss, were considered important monitoring parameters, allowing a better understanding of the sys-temic human disease. Mice were inoculated subcutaneously and evaluated by the percentage weight variation as well as the clinical signs. Hematological parameters and cytokines levels were measured and viral titration in brain tissue or serum neutralization was performed to confirm mice infection. The subcutaneously DENV inoculated mice showed weight loss after infection, but they did not show any other clinical signs. The leukocytes and platelets de-creased after subcutaneous inoculation. The cytokines TNF alpha and IFN gamma increased after infection in mice. The subcutaneous model provided scope for improved understanding of the dengue pathogenesis, as well as possible mechanism for protection to subsequent mouse infected by intra-cranial route in mice. This model could be used to study the vertebrate im-mune response and evaluation of drugs or vaccine against dengue virus. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Departamento de Microbiologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil / Empresa Brasileira de Pesquisa Agropecuária. Laboratório de Virologia. Brasilia, DF, Brasil. | pt_BR |
| Affilliation | Fundacao Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Microbiologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Subject | Subcutaneous Murine Model | pt_BR |
| Subject | Dengue Disease | pt_BR |
| Subject | Immune Competent Mouse | pt_BR |
| Subject | Cytokines | pt_BR |
| Subject | Innate Immune Response | pt_BR |
| Embargo date | 2025-01-01 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
