| Author | Santoro, Tassia | |
| Author | Azevedo, Carolina T. | |
| Author | Silva, Patrícia M. R. e | |
| Author | Martins, Marco A. | |
| Author | Carvalho, Vinicius F. | |
| Access date | 2019-05-02T11:55:20Z | |
| Available date | 2019-05-02T11:55:20Z | |
| Document date | 2019 | |
| Citation | SANTORO, Tassia et al. Glucocorticoids decrease the numbers and activation ofmast cells by inducing the transactivation receptors of AGEs. J Leukoc Biol., v. 105, p.131-142, 2019. | pt_BR |
| ISSN | 0741-5400 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/32901 | |
| Language | eng | pt_BR |
| Publisher | Society for Leukocyte Biology | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Mastócitos | pt_BR |
| Subject in Portuguese | Alergia | pt_BR |
| Subject in Portuguese | Apoptose | pt_BR |
| Title | Glucocorticoids decrease the numbers and activation ofmast cells by inducing the transactivation receptors of AGEs | pt_BR |
| Type | Article | |
| Abstract | Glucocorticoids (GCs) are potent anti-allergic compounds that function, at least in part, by inhibiting
signaling pathways in mast cells. We hypothesized that the GC-induced mastocytopenia
and suppression of mast cell activation are mediated by the advanced glycation end products
(AGEs)/receptors of AGEs (RAGEs) signaling axis.We evaluated the role of AGEs in GC-mediated
mastocytopenia and impaired mast cell degranulation in male Wistar rats and Swiss–Webster
mice subcutaneously injected with dexamethasone or prednisolone (0.1 mg/kg) once a day for
21 consecutive days. The animals were treated with either the AGE inhibitor aminoguanidine
(250 mg/kg), the RAGE antagonist FPS-ZM1 (1 mg/kg) or the galectin-3 antagonist GSC-100
(1 mg/kg) daily for 18 days, starting 3 days following GC treatment. Aminoguanidine inhibited
GC-induced mast cell apoptosis and restored mast cell numbers in the pleural cavity of GC-treated
rats. Aminoguanidine also reversed the GC-induced reduction in histamine release triggered by
allergens or compound 48/80 in vitro. GC treatment induced RAGE and galectin expression in
mast cells, and blocking these agents by FPS-ZM1 or GSC-100 significantly reversed mast cell
numbers in the peritoneal cavity and mesenteric tissue of GC-treated mice. In addition, the
combination of GC and AGE-induced mast cell apoptosis in vitro was inhibited by both FPS-ZM1
and GSC-100. We concluded that the GC-induced mastocytopenia and suppression of mast cell
stimulation are associated with the gene transactivation of RAGE and galectin-3. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Basil. | pt_BR |
| Subject | Mast cells | pt_BR |
| Subject | Allergy | pt_BR |
| Subject | Apoptosis | pt_BR |
| e-ISSN | 1938-3673 | |
| Embargo date | 2022-01-01 | |
