| Author | Rodrigues, Carolina Rego | |
| Author | Ferreira, André Teixeira | |
| Author | Vargas, Frey Francisco Romero | |
| Author | Duarte, Clara Guerra | |
| Author | Oliveira, Fernanda Costal | |
| Author | Stransky, Stephanie | |
| Author | Souza, Letícia Lopes de | |
| Author | Dutra, Alexandre Augusto Assis | |
| Author | Yarlequé, Armando | |
| Author | Bonilla, César | |
| Author | Sanchez, Eladio Flores | |
| Author | Perales, Jonas | |
| Author | Chávez-Olórtegui, Carlos | |
| Access date | 2019-05-07T13:03:03Z | |
| Available date | 2019-05-07T13:03:03Z | |
| Document date | 2018 | |
| Citation | RODRIGUES, Carolina Rego et al. Proteomic profile, biological activities and antigenic analysis of the venom from Bothriopsis bilineata smaragdina (“loro machaco”), a pitviper snake from Peru. Journal of Proteomics, v. 187, p.171-181, July 2018. | pt_BR |
| ISSN | 1874-3919 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/32974 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Proteômica de veneno | pt_BR |
| Subject in Portuguese | Caracterização de veneno | pt_BR |
| Subject in Portuguese | Cobra peruana | pt_BR |
| Subject in Portuguese | Bothriopsis bilineata smaragdina | pt_BR |
| Title | Proteomic profile, biological activities and antigenic analysis of the venom from Bothriopsis bilineata smaragdina ("loro machaco"), a pitviper snake from Peru | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.jprot.2018.07.016 | |
| Abstract | In order to determine Bothriopsis bilineata smaragdina venom (BbsV) composition, proteomic approaches were performed. Venom components were analyzed by RP-HPLC, SDS- PAGE and nano LC on line with LTQ Orbitrap XL. Results showed a total of 189 identified proteins, grouped into 11 different subgroups, which include snake venom metalloproteinases (SVMPs, 54.67%), snake C-type lectins (Snaclecs, 15.78%), snake venom serine proteinases (SVSPs, 14.69%), cystein-rich secretory proteins (CRISP, 2.61%), phospholipases A2 (PLA2, 1.14%), phosphodiesterase (PDE, 1.17%), venom endothelial growth factor (VEGF, 1.06%) 5'nucleotidases (0.33%), L-amino acid oxidases (LAAOs, 0.28%) and other proteins. In vitro enzymatic activities (SVMP, SVSP, LAAO, Hyal and PLA2) of BbsV were also analyzed. BbsV showed high SVSP activity but low PLA2 activity, when compared to other Bothrops venoms. In vivo, BbsV induced hemorrhage and edema in mice and showed intraperitoneal median lethal dose (LD50) of 92.74 (± 0.15) μg/20 g of mice. Furthermore, BbsV reduced cell viability when incubated with VERO cells. Peruvian and Brazilian bothropic antivenoms recognize BbsV proteins, as detected by ELISA and Western Blotting. Both antivenoms were able to neutralize in vivo edema and hemorrhage. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidad Nacional Jorge Basadre Grohmann. Tacna, Peru. | pt_BR |
| Affilliation | Fundação Ezequiel Dias. Centro de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal dos Vales do Jequitinhonha e Mucuri. Faculdade de Medicina do Mucuri. Teófilo Otoni, MG, Brasil. | pt_BR |
| Affilliation | Universidad Nacional Mayor de San Marcos. Lima, Peru. | pt_BR |
| Affilliation | Instituto Nacional de Salud. Lima, Peru. | pt_BR |
| Affilliation | Fundação Ezequiel Dias. Centro de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Subject | Bothriopsis bilineata smaragdina | pt_BR |
| Subject | Venom proteomics | pt_BR |
| Subject | Peruvian snake | pt_BR |
| Subject | Venom characterization | pt_BR |
| e-ISSN | 1876-7737 | |
| Embargo date | 2022-01-01 | |
