| Author | Cardoso, Thiago | |
| Author | Bezerra, Caroline | |
| Author | Medina, Lilian Silva | |
| Author | Ramasawmy, Rajendranath | |
| Author | Scheriefer, Albert | |
| Author | Bacellar, Olívia | |
| Author | Carvalho Filho, Edgar Marcelino | |
| Access date | 2019-05-10T16:39:44Z | |
| Available date | 2019-05-10T16:39:44Z | |
| Document date | 2019 | |
| Citation | CARDOSO, T. et al. Leishmania braziliensis isolated from disseminated leishmaniasis patients downmodulate neutrophil function. Parasite Immunology, v. 41, p. 1-8, 2019. | pt_BR |
| ISSN | 0141-9838 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/33037 | |
| Sponsorship | National
Institutes of Health (NIH) grant U01‐
AI136032 and the National Institute of
Tropical Diseases (INCT‐DT). | pt_BR |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Leishmaniose Cutânea | pt_BR |
| Subject in Portuguese | Leishmaniose disseminada | pt_BR |
| Subject in Portuguese | Leishmania braziliensis | pt_BR |
| Subject in Portuguese | Neutrófilos | pt_BR |
| Title | Leishmania braziliensis isolated from disseminated leishmaniasis patients downmodulate neutrophil function | pt_BR |
| Type | Article | |
| DOI | 10.1111/pim.12620 | |
| Abstract | The polymorphism observed in Leishmania braziliensis is associated with different clinical forms of leishmaniasis. Neutrophils (PMNs) participate in the pathogenesis of leishmania infection, and here, we evaluate neutrophil function after infection with isolates of L. braziliensis from cutaneous leishmaniasis (CL) or disseminated leishmaniasis (DL) patients. Methods and results: Neutrophils from 30 healthy subjects (HS) were infected with
isolates of L. (V.) braziliensis obtained from three CL and three DL patients. They were
infected at the ratio of 3:1 parasites per neutrophil, and leishmania uptake was evaluated
by microscopy. The neutrophil activation markers and oxidative burst by expression
of dihidrorhodamine (DHR) were evaluated by flow cytometry and cytokine
production by ELISA. The frequency of infected cells and the number of amastigotes
were higher in neutrophils infected with CL isolates compared to DL isolates
(P < 0.05). The DHR and CD66b expression after infection with DL isolate was lower
than with CL isolates. There was no difference regarding chemokine production.
Conclusion: The L. (V.) braziliensis isolates of DL induced lower respiratory burst and
neutrophils activation markers compared with CL isolates which may contribute to
parasite survival and dissemination in DL patients. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Pesquisas Clinicas. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / The National Institute of Science and Technology in Tropical Diseases. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / The National Institute of Science and Technology in Tropical Diseases. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Pesquisas Clinicas. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / The National Institute of Science and Technology in Tropical Diseases. Salvador, BA, Brasil. | pt_BR |
| Subject | Cutaneous leishmaniasis | pt_BR |
| Subject | Disseminated leishmaniasis, | pt_BR |
| Subject | Leishmania braziliensis | pt_BR |
| Subject | Neutrophils | pt_BR |
