| Author | Polari, Ludmila Porto Mendes | |
| Author | Carneiro, Pedro Paulo Oliveira | |
| Author | Macedo, Michael Nascimento | |
| Author | Machado, Paulo Roberto Lima | |
| Author | Scott, Phillip | |
| Author | Carvalho Filho, Edgar Marcelino | |
| Author | Bacellar, Maria Olívia Amado Ramos | |
| Access date | 2019-05-14T14:29:37Z | |
| Available date | 2019-05-14T14:29:37Z | |
| Document date | 2019 | |
| Citation | POLARI, Ludmila Porto Mendes et al. Leishmania braziliensis Infection Enhances Toll-Like Receptors 2 and 4 Expression and Triggers TNF-α and IL-10 Production in Human Cutaneous Leishmaniasis. Frontiers in Cellular and Infection Microbiology, v. 9, n. 120, p. 1-11, May 2019. | pt_BR |
| ISSN | 2235-2988 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/33061 | |
| Sponsorship | National Institute of Health
(NIH) grant AI136032. The funders had no role in study design,
data collection and analysis, decision to publish, or preparation
of the manuscript. | pt_BR |
| Language | eng | pt_BR |
| Publisher | Frontiers Media | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Leishmaniose tegumentar humana | pt_BR |
| Subject in Portuguese | Leishmania braziliensis | pt_BR |
| Subject in Portuguese | Toll like receptor | pt_BR |
| Subject in Portuguese | Toll like receptor 4 | pt_BR |
| Subject in Portuguese | Inflamação | pt_BR |
| Subject in Portuguese | Subconjuntos de monócitos | pt_BR |
| Title | Leishmania braziliensis Infection Enhances Toll-Like Receptors 2 and 4 Expression and Triggers TNF-α and IL-10 Production in Human Cutaneous Leishmaniasis | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.3389/fcimb.2019.00120 | pt_BR |
| Abstract | Cutaneous leishmaniasis (CL) caused by infection with Leishmania braziliensis is
characterized by an exaggerated inflammatory response that controls the parasite
burden, but also contributes to pathology. While myeloid cells are required to eliminate
the parasite, recent studies indicate that they may also participate in the inflammatory
response driving disease progression. The innate immune response to leishmania is
driven in part by the Toll-like receptors (TLRs) TLR2, TLR4, and TLR9. In this study,
we used flow cytometric analysis to compare TLR2 and TLR4 expression in monocyte
subsets (classical, intermediate, and non-classical) fromCL patients and healthy subjects
(HS). We also determined if there was an association of either the pro-inflammatory
cytokine TNF or the anti-inflammatory cytokine IL-10 with TLR2 or TLR4 expression levels
after L. braziliensis infection. In vitro infection with L. braziliensis caused CL monocytes
to up-regulate TLR2 and TLR4 expression. We also found that intermediate monocytes
expressed the highest levels of TLR2 and TLR4 and that infected monocytes produced
more TNF and IL-10 than uninfected monocytes. Finally, while classical and intermediate
monocytes were mainly responsible for TNF production, classical monocytes were
the main source of IL-10. Collectively, our studies revealed that up-regulated TLR2/4
expression and TNF production by intermediate/inflammatory subsets of monocytes
from patients correlates with detrimental outcome of cutaneous leishmaniasis. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brazil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brazil. | pt_BR |
| Affilliation | University of Pennsylvania. School of Veterinary Medicine. Department of Pathobiology. Philadelphia, PA, United States. | pt_BR |
| Affilliation | Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brazil. | pt_BR |
| Subject | Human cutaneous leishmaniasis | pt_BR |
| Subject | Leishmania braziliensis | pt_BR |
| Subject | Toll like receptor 2 | pt_BR |
| Subject | Toll like receptor 4 | pt_BR |
| Subject | Inflammation | pt_BR |
| Subject | Monocytes subsets | pt_BR |
