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ANTILEISHMANIAL ACTIVITY OF DIMERIC FLAVONOIDS ISOLATED FROM ARRABIDAEA BRACHYPODA
Autor
Afiliación
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Engenharia Tecidual e e Imunofarmacologia. Salvador, BA, Brasil.
Universidade Federal do Maranhão. Departamento de Química. São Luiz, MA, Brasil.
University of Geneva. University of Lausanne. School of Pharmaceutical Sciences. Geneva, Switzerland.
University of Geneva. University of Lausanne. School of Pharmaceutical Sciences. Geneva, Switzerland.
Universidade do Estado de São Paulo. Campus Experimental do Litoral Paulista. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Engenharia Tecidual e e Imunofarmacologia. Salvador, BA, Brasil.
University of Geneva. University of Lausanne. School of Pharmaceutical Sciences. Geneva, Switzerland.
University of Geneva. University of Lausanne. School of Pharmaceutical Sciences. Geneva, Switzerland.
University of Geneva. University of Lausanne. School of Pharmaceutical Sciences. Geneva, Switzerland.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Engenharia Tecidual e e Imunofarmacologia. Salvador, BA, Brasil.
Universidade Federal do Maranhão. Departamento de Química. São Luiz, MA, Brasil.
University of Geneva. University of Lausanne. School of Pharmaceutical Sciences. Geneva, Switzerland.
University of Geneva. University of Lausanne. School of Pharmaceutical Sciences. Geneva, Switzerland.
Universidade do Estado de São Paulo. Campus Experimental do Litoral Paulista. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Engenharia Tecidual e e Imunofarmacologia. Salvador, BA, Brasil.
University of Geneva. University of Lausanne. School of Pharmaceutical Sciences. Geneva, Switzerland.
University of Geneva. University of Lausanne. School of Pharmaceutical Sciences. Geneva, Switzerland.
University of Geneva. University of Lausanne. School of Pharmaceutical Sciences. Geneva, Switzerland.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Engenharia Tecidual e e Imunofarmacologia. Salvador, BA, Brasil.
Resumen en ingles
Leishmaniasis are diseases caused by parasites belonging to Leishmania genus. The treatment with pentavalent antimonials present high toxicity. Secondary line drugs, such as amphotericin B and miltefosine also have a narrow therapeutic index. Therefore, there is an urgent need to develop new drugs to treat leishmaniasis. Here, we present the in vitro anti-leishmanial activity of unusual dimeric flavonoids purified from Arrabidaea brachypoda. Three compounds were tested against Leishmana sp. Compound 2 was the most active against promastigotes. Quantifying the in vitro infected macrophages revealed that compound 2 was also the most active against intracellular amastigotes of L. amazonensis, without displaying host cell toxicity. Drug combinations presented an additive effect, suggesting the absence of interaction between amphotericin B and compound 2. Amastigotes treated with compound 2 demonstrated alterations in the Golgi and accumulation of vesicles inside the flagellar pocket. Compound 2-treated amastigotes presented a high accumulation of cytoplasmic vesicles and a myelin-like structure. When administered in L. amazonensis-infected mice, neither the oral nor the topical treatments were effective against the parasite. Based on the high in vitro activity, dimeric flavonoids can be used as a lead structure for the development of new molecules that could be useful for structure-active studies against Leishmania.
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