| Author | Marcili, A. | |
| Author | Lima, L. | |
| Author | Cavazzana, M. | |
| Author | Junqueira, A. C. V. | |
| Author | Veludo, H. H. | |
| Author | Silva, F. Maia da | |
| Author | Campaner, M. | |
| Author | Paiva, F. | |
| Author | Nunes, V. L. B. | |
| Author | Teixeira, M. M. G. | |
| Access date | 2019-05-16T11:58:38Z | |
| Available date | 2019-05-16T11:58:38Z | |
| Document date | 2009 | |
| Citation | MARCILI, A. et al. A new genotype of Trypanosoma cruzi associated with bats evidenced by phylogenetic analyses using SSU rDNA, cytochrome b and Histone H2B genes and genotyping based on ITS1 rDNA. Parasitology, v. 136, p. 641-655, 2009. | pt_BR |
| ISSN | 0031-1820 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/33102 | |
| Language | eng | pt_BR |
| Publisher | Cambridge University Press | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Linhagens de Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Doença de Chagas | pt_BR |
| Subject in Portuguese | Genotipagem | pt_BR |
| Subject in Portuguese | Filogenia | pt_BR |
| Subject in Portuguese | Evolução | pt_BR |
| Subject in Portuguese | Parasitas de morcego | pt_BR |
| Subject in Portuguese | Citocromo b | pt_BR |
| Subject in Portuguese | Quirópteros | pt_BR |
| Title | A new genotype of Trypanosoma cruzi associated with bats evidenced by phylogenetic analyses using SSU rDNA, cytochrome b and Histone H2B genes and genotyping based on ITS1 rDNA | pt_BR |
| Type | Article | |
| DOI | 10.1017/S0031182009005861 | |
| Abstract | We characterized 15 Trypanosoma cruzi isolates from bats captured in the Amazon, Central and Southeast Brazilian regions. Phylogenetic relationships among T. cruzi lineages using SSU rDNA, cytochrome b, and Histone H2B genes positioned all Amazonian isolates into T. cruzi I (TCI). However, bat isolates from the other regions, which had been genotyped as T. cruzi II (TC II) by the traditional genotyping method based on mini-exon gene employed in this study, were not nested within any of the previously defined TCII sublineages, constituting a new genotype designated as TCbat. Phylogenetic analyses demonstrated that TCbat indeed belongs to T. cruzi and not to other closely related bat trypanosomes of the subgenus Schizotrypanum, and that although separated by large genetic distances TCbat is closest to lineage TCI. A genotyping method targeting ITS1 rDNA distinguished TCbat from established T. cruzi lineages, and from other Schizotrypanum species. In experimentally infected mice, TCbat lacked virulence and yielded low parasitaemias. Isolates of TCbat presented distinctive morphological features and behaviour in triatomines. To date, TCbat genotype was found only in bats from anthropic environments of Central and Southeast Brazil. Our findings indicate that the complexity of T. cruzi is larger than currently known, and confirmed bats as important reservoirs and potential source of T. cruzi infections to humans. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil / Faculdade de Medicina de Catanduva. Catanduva, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal de Rondônia. Porto Velho, RO, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Mato Grosso do Sul. Departamento de Parasitologia Veterinária. Campo Grande, MS, Brasil. | pt_BR |
| Affilliation | Universidade para o Desenvolvimento do Estado e da Região do Pantanal. Centro de Ciências Biológicas, Agrárias e da Saúde. Campo Grande, MS, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil. | pt_BR |
| Subject | Trypanosoma cruzi lineages | pt_BR |
| Subject | Chagas Disease | pt_BR |
| Subject | Chiroptera | pt_BR |
| Subject | Genotyping | pt_BR |
| Subject | Phylogeny | pt_BR |
| Subject | Evolution | pt_BR |
| Subject | Bat parasites | pt_BR |
| Subject | SSU rDNA | pt_BR |
| Subject | Cytochrome b | pt_BR |
| Subject | Histone H2B | pt_BR |
| DeCS | Quirópteros | pt_BR |
| e-ISSN | 1469-8161 | |
| Embargo date | 2022-01-01 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
