| Author | Lacerda, G. R. da Silva | |
| Author | Melo, C. M. L. de | |
| Author | Soares, A. K. de Araújo | |
| Author | Moreira, L. R. | |
| Author | Coriolano, M. C. | |
| Author | Lima, G. M. de Souza | |
| Author | Napoleão, T. H. | |
| Author | Lorena, V. M. B.de | |
| Author | Silva, L. A. de Oliveira da | |
| Author | Nascimento, S. C. do | |
| Access date | 2019-05-22T16:08:10Z | |
| Available date | 2019-05-22T16:08:10Z | |
| Document date | 2018 | |
| Citation | SILVA, G. R. da Silva et al. L-Asparaginase Isolated from Streptomyces Ansochromogenes Promotes Th1 Profile and Activates CD8+ T Cells in Human PBMC: An in Vitro Investigation. Journal of Applied Microbiology, v. 124, n. 5, p. 1122–1130, May 2018. | pt_BR |
| ISSN | 1365-2672 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/33180 | |
| Language | eng | pt_BR |
| Rights | restricted access | pt_BR |
| Title | L-asparaginase isolated from Streptomyces ansochromogenes promotes Th1 profile and activates CD8+ T cells in human PBMC: an in vitro investigation | pt_BR |
| Type | Article | |
| DOI | 10.1111/jam.13644 | |
| Abstract | Aims:A new L-asparaginase produced byStreptomyces ansochromogenesUFPEDA 3420 actinobacteria was used in this study against humanlymphocyte cultures to evaluate the immunological profile induced by thisenzyme.Methods and Results:Cultures of lymphocytes were stimulated withS.ansochromogenesL-asparaginase, and cytotoxicity, cell viability, cell stimulationand cytokine production were analysed. This newS. ansochromogenesL-asparaginase induced activation and proliferation of the TCD8+lymphocytesubset and produced higher TNF-a, IFN-c, IL-2 and IL-10 levels in a 24-hassay.Conclusion:Streptomyces ansochromogenesL-asparaginase is a promisingmolecule to be used inin vivomodels and to deepen preclinical tests againstacute lymphoblast leukaemia.Significance and Impact of Study:L-asparaginase is an indispensablecomponent of the chemotherapeutic treatment of acute lymphoblast leukaemia(ALL) and acute myeloid leukaemia (AML). Currently, drugs such asAsparaginaseâ, Kidrolaseâ, and Elsparâand Erwinaseâare efficient againstleukemic disease, but promote immunosuppression and other side effects inhuman organisms. Our purifiedS. ansochromogenesL-asparaginase showedpromissory results inducing,in vitro, higher immunostimulation in humanPBMC, especially in T CD8+lymphocyte subsets. | pt_BR |
| Affilliation | Federal University of Pernambuco. Department of Antibiotics. Recife, PE, Brazil. | pt_BR |
| Affilliation | Federal University of Pernambuco. Department of Antibiotics. Laboratory of Immunological and Antitumor Analyzes. Recife, PE, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Imunoparasitologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Imunoparasitologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Federal University of Pernambuco. Department of Biochemistry. Laboratory of Glycoproteins. Recife, PE, Brasil. | pt_BR |
| Affilliation | Federal University of Pernambuco. Department of Antibiotics. Recife, PE, Brazil. | pt_BR |
| Affilliation | Federal University of Pernambuco. Department of Biochemistry. Laboratory of Glycoproteins. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Imunoparasitologia. | pt_BR |
| Affilliation | Federal University of Pernambuco. Department of Antibiotics. Recife, PE, Brazil. | pt_BR |
| Affilliation | Federal University of Pernambuco. Department of Antibiotics. Recife, PE, Brazil. | pt_BR |
| Subject | Acute lymphoblast leukaemia | pt_BR |
| Subject | Immunomodulation | pt_BR |
| Subject | L-asparaginase | pt_BR |
| Subject | Lymphocytes | pt_BR |
| Subject | Streptomyces ansochromogenes | pt_BR |
| DeCS | Asparaginase / isolamento e purificação | pt_BR |
| DeCS | Asparaginase / farmacologia | pt_BR |
| DeCS | Asparaginase / toxicidade | pt_BR |
| DeCS | Linfócitos T CD8-Positivos / efeitos de drogas | pt_BR |
| DeCS | Linfócitos T CD8-Positivos / imunologia | pt_BR |
| DeCS | Humanos | pt_BR |
| DeCS | Leucócitos Mononucleares / efeitos de drogas | pt_BR |
| DeCS | Leucócitos Mononucleares / imunologia | pt_BR |
| DeCS | Ativação de Linfócitos / efeitos de drogas | pt_BR |
| DeCS | Streptomyces / enzimologia | pt_BR |
| DeCS | Th1 Células / efeitos de drogas | pt_BR |
| Embargo date | 2050-01-01 | |
