| Author | Almeida-Paes, Rodrigo | |
| Author | Pimenta, Monique A. | |
| Author | Pizzini, Claudia Vera | |
| Author | Monteiro, Paulo Cezar F. | |
| Author | Peralta, José Mauro | |
| Author | Nosanchuk, Joshua D. | |
| Author | Zancopé-Oliveira, Rosely Maria | |
| Access date | 2019-06-13T14:08:18Z | |
| Available date | 2019-06-13T14:08:18Z | |
| Document date | 2007 | |
| Citation | ALMEIDA-PAES, Rodrigo et al. Use of mycelial-phase Sporothrix schenckii exoantigens in an enzyme-linked immunosorbent assay for diagnosis of sporotrichosis by antibody detection. Clinical and Vaccine Immunology, v. 14, n. 3, p. 244-249, Mar. 2007. | pt_BR |
| ISSN | 1556-6811 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/33493 | |
| Language | eng | pt_BR |
| Publisher | American Society for Microbiology | pt_BR |
| Rights | open access | pt_BR |
| Title | Use of mycelial-phase Sporothrix schenckii exoantigens in an enzyme-linked immunosorbent assay for diagnosis of sporothricosis by antibody detection | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1128/CVI.00430-06 | |
| Abstract | An enzyme-linked immunosorbent assay (ELISA) was developed for specific antibody detection in serum
specimens of patients with sporotrichosis. The assay was made with mycelial-phase Sporothrix schenckii
exoantigens and was tested against 90 sera from patients with different clinical forms of sporotrichosis. Potential cross-reactions were analyzed with 72 heterologous sera from patients with paracoccidioidomycosis,
cryptococcosis, aspergillosis, histoplasmosis, tuberculosis, and American tegumentary leishmaniasis, as well as 76 sera from healthy controls. We found a sensitivity of 97% and a specificity of 89% in this assay. Some
cross-reactions were seen, as observed in other immunoassays for the diagnosis of sporotrichosis. The ELISA
appears to be especially useful for cutaneous forms of disease, since these are not promptly diagnosed with
available immunoprecipitation or agglutination techniques. These results suggest that the ELISA using
mycelial-phase S. schenckii exoantigens is a very sensitive diagnostic tool for the serodiagnosis of sporotrichosis and can be used in conjunction with conventional methods of diagnosis, particularly in cases where crossreactions or false-positive results are experienced with the serodiagnosis. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Micologia. Rio de Janeiro, RJ, Brasil / Albert Einstein College of Medicine. Department of Microbiology and Immunology. New York, NY, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Micologia. Rio de Janeiro, RJ. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Micologia. Rio de Janeiro, RJ. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Micologia. Rio de Janeiro, RJ. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Albert Einstein College of Medicine. Department of Medicine. Division of Infectious Diseases. New York, NY, USA / Albert Einstein College of Medicine. Department of of Microbiology and Immunology. New York, NY, USA | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Micologia. Rio de Janeiro, RJ. | pt_BR |
| Subject | Sporothrix schenckii | pt_BR |
| Subject | Mycelial-phase | pt_BR |
| Subject | Sporotrichosis | pt_BR |
| Subject | Exoantigens | pt_BR |
| Subject | ELISA | pt_BR |
| e-ISSN | 1556-679X | |
| Embargo date | 2019-12-13 | |
