| Author | Guedes, H. L. | |
| Author | Guimarães, A. J. | |
| Author | Muniz, M. M. | |
| Author | Pizzini, C. V. | |
| Author | Hamilton, A. J. | |
| Author | Peralta, J. M. | |
| Author | Deepe, G. S. | |
| Author | Zancopé-Oliveira, Rosely M. | |
| Access date | 2019-06-18T12:49:06Z | |
| Available date | 2019-06-18T12:49:06Z | |
| Document date | 2003 | |
| Citation | GUEDES, Herbert Leonel de Matos et al. PCR assay for identification of histoplasma capsulatum based on the nucleotide sequence of the M antigen. Journal of Clinical Microbiology, v. 41, n. 2, p. 535-539, Feb. 2003. | pt_BR |
| ISSN | 0095-1137 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/33537 | |
| Language | eng | pt_BR |
| Publisher | American Society for Microbiology | pt_BR |
| Rights | open access | pt_BR |
| Title | PCR assay for identification of histoplasma capsulatum based on the nucleotide sequence of the M antigen | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1128/JCM.41.2.535-539.2003 | |
| Abstract | The major diagnostic antigens of Histoplasma capsulatum var. capsulatum are the H and M antigens, pluripotent glycoproteins that elicit both humoral and T-cell-mediated immune responses. The gene encoding
the M antigen has previously been sequenced, and its sequence has significant overall homology to those of the genes for fungal catalases. Regions of the M-antigen gene with little or no homology were used to design four oligonucleotide sequences for application in the PCR detection and identification of H. capsulatum var.
capsulatum. The PCR correctly identified the 31 H. capsulatum var. capsulatum strains isolated from human,
animal, and soil specimens and 1 H. capsulatum var. duboisii isolate. PCR products of 111 and 279 bp were
amplified with primers Msp1F-Msp1R and Msp2F-Msp2R, respectively. No amplification product was obtained from DNA extracted from an H. capsulatum var. farciminosum isolate. The specificity of the PCR with the
M-antigen-derived primers was confirmed by the total absence of amplification products when genomic DNA
from Paracoccidioides brasiliensis, Candida spp., Sporothrix schenckii, Cryptococcus neoformans, Blastomyces dermatitidis, Coccidioides immitis, Aspergillus niger, and Aspergillus fumigatus were applied in the reaction. This rapid, sensitive, and specific assay provides a way to identify typical and atypical isolates of H. capsulatum var. capsulatum. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Kings College. Guys Hospital. St. Johns Institute of Dermatology. London, United Kingdom. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | University of Cincinnati. College of Medicine. Cincinnati, OH, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Histoplasma capsulatum | pt_BR |
| Subject | M antigen | pt_BR |
| Subject | PCR | pt_BR |
| e-ISSN | 1098-660X | |
| Embargo date | 2019-12-18 | |
