| Author | Castro, Ana Cristina Honorato de | |
| Author | Kochi, Leandro Toshio | |
| Author | Moço, Anna Clara Rios | |
| Author | Coimbra, Roney Santos | |
| Author | Oliveira, Guilherme Corrêa de | |
| Author | Cuadros-Orellana, Sara | |
| Author | Madurro, João Marcos | |
| Author | Brito-Madurro, Ana Graci | |
| Access date | 2019-06-25T19:11:22Z | |
| Available date | 2019-06-25T19:11:22Z | |
| Document date | 2018 | |
| Citation | CASTRO, Ana Cristina Honorato de et al. A new genosensor for meningococcal meningitis diagnosis using biological samples. Journal of Solid State Electrochemistry, v. 22, n. 8, p. 2339-2346, 2018. | pt_BR |
| ISSN | 1432-8488 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/33668 | |
| Language | eng | pt_BR |
| Publisher | Springer Verlag | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Biosensor | pt_BR |
| Subject in Portuguese | Menigite | pt_BR |
| Title | A new genosensor for meningococcal meningitis diagnosis using biological samples | pt_BR |
| Type | Article | |
| DOI | 10.1007/s10008-018-3940-0 | |
| Abstract | In this work, a new electrochemical biosensor for DNA detection of bacterial meningitis is proposed. The system is based on specific DNA fragments from the Neisseria meningitidis genome as a probe incorporated on graphite electrodes modified with poly(4-aminophenol). Detection of a complementary oligonucleotide sequence, a specific 710-base pair amplicon, and the genomic DNA of bacteria was carried out by differential pulse voltammetry, using ethidium bromide as an electroactive indicator of hybridization. The complementary oligonucleotide and the genomic DNA of Neisseria meningitidis were quantified by the genosensor, showing detection limits of 0.6 ng mu L-1 and about 6 ng mu L-1, respectively. Morphological differences were observed between hybridized and unhybridized surfaces by atomic force microscopy. The biosensor showed high selectivity, discriminating non-specific targets, and high stability retaining over 98% of its original activity after 120 days of storage. The bioelectrode was effective in discriminating the genomic DNA in samples with human serum without significant interference, proving to be an interesting platform for meningococcal meningitis diagnosis. | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Instituto de Biotecnologia. Uberlândia, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Instituto de Biotecnologia. Uberlândia, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Instituto de Biotecnologia. Uberlândia, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Centro de Excelência em Bioinformática. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Vale Instituto de Technologia. Belém, PA, Brasil. | pt_BR |
| Affilliation | Universidad Católica del Maule. Department of Forestry Sciences.Talca, Chile. | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Instituto de Química. Uberlândia, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Instituto de Biotecnologia. Uberlândia, MG, Brasil. | pt_BR |
| Subject | Biosensor | pt_BR |
| Subject | Meningitidis | pt_BR |
| Subject | Modified electrode | pt_BR |
| Subject | Poly (4-aminophenol) | pt_BR |
| Embargo date | 2025-01-01 | |
