| Author | Nunes, Rodrigo Dutra | |
| Author | Oliveira, Ricardo Lourenço de | |
| Author | Braz, Glória Regina Cardoso | |
| Access date | 2019-06-28T11:02:36Z | |
| Available date | 2019-06-28T11:02:36Z | |
| Document date | 2008 | |
| Citation | NUNES, Rodrigo Dutra et al. A novel method for measuring fructose ingestion by mosquitoes. Journal of Vector Ecology, v. 33, n. 2, p. 224-231, 2008. | pt_BR |
| ISSN | 1081-1710 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/33699 | |
| Language | eng | pt_BR |
| Publisher | Society for Vector Ecology | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Aedes aegypti | pt_BR |
| Subject in Portuguese | Aedes albopictus | pt_BR |
| Subject in Portuguese | Frutose | pt_BR |
| Subject in Portuguese | Alimentação de açúcar | pt_BR |
| Subject in Portuguese | Metabolismo | pt_BR |
| Title | A novel method for measuring fructose ingestion by mosquitoes | pt_BR |
| Type | Article | |
| DOI | 10.3376/1081-1710-33.2.225 | |
| Abstract | We adapted the Seliwanoff method to quantify fructose in mosquitoes. This method showed a minimum
detection limit of 2.4 μg of fructose, and was more reliable and nearly four times more sensitive than the anthrone test. The
Seliwanoff method was used to measure the maximum sugar intake by individual mosquitoes and to determine the digestion
time of this nutrient by both Aedes aegypti and Aedes albopictus in the laboratory. Sugar intake by Ae. albopictus was up to 1.7
times higher than that of Ae. aegypti. The amount of sugar ingested by females was up to 2.5 times higher than that of males
in both species. After 48 h, a fructose meal was not detected any longer in either species. The Seliwanoff method was applied
to measure fructose content of field-collected Ae. aegypti males and females in Rio de Janeiro. Results showed that even Ae.
aegypti females do feed on sugars. The standardized Seliwanoff method proved to be reliable for measuring the sugar content
of individual mosquitoes and can be used wherever estimation of small quantities of fructose is needed. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Laboratório de Bioquímica de Vetores de Doenças. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Laboratório de Bioquímica de Vetores de Doenças. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Aedes aegypti | pt_BR |
| Subject | Aedes albopictus | pt_BR |
| Subject | Fructose | pt_BR |
| Subject | Sugar feeding | pt_BR |
| Subject | Metabolism | pt_BR |
| e-ISSN | 1948-7134 | |
| Embargo date | 2022-01-01 | |
| xmlui.metadata.dc.subject.ods | 02 Fome zero e agricultura sustentável | |
