| Author | Figueiredo, Fabiano Borges | |
| Author | Vasconcelos, Tassia Cristina Bello de | |
| Author | Madeira, Maria de Fátima | |
| Author | Menezes, Rodrigo Caldas | |
| Author | Maia-Elkhoury, Ana Nilce Silveira | |
| Author | Marcelino, Andreza Pain | |
| Author | Werneck, Guilherme L. | |
| Access date | 2019-06-28T19:44:44Z | |
| Available date | 2019-06-28T19:44:44Z | |
| Document date | 2018 | |
| Citation | FIGUEIREDO, Fabiano Borges et al. Validation of the Dual-path Platform chromatographic immunoassay (DPP® CVL rapid test) for the serodiagnosis of canine visceral leishmaniasis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 11, p. 1-7, 2018. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/33735 | |
| Language | eng | pt_BR |
| Publisher | Ministério da Saúde. Instituto Oswaldo Cruz | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Teste rápido CVL de plataforma de caminho duplo | pt_BR |
| Title | Validation of the Dual-path Platform chromatographic immunoassay (DPP® CVL rapid test) for the serodiagnosis of canine visceral leishmaniasis | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1590/0074-02760180260 | |
| Abstract | Visceral leishmaniasis is a major public health challenge in South America, and dogs are its main urban reservoir. It has the objective to do the validation of the canine Dual-path Platform immunoassay for canine visceral leishmaniasis (DPP® CVL) for a sample set composed of 1446 dogs from different Brazilian endemic areas. It had as methods a well-defined reference standard by means of parasitological culture, immunohistochemistry, and histopathology was used. Animals were classified as asymptomatic, oligosymptomatic, or symptomatic. Sensitivity and specificity were assessed as a single set and in clinical groups. A reproducibility assessment of the tests was conducted using the Kappa (κ) index at three different laboratories (A, B, and C). It was findings that overall, 89% sensitivity and 70% specificity were obtained for the entire sample set. Analysis of the clinical groups showed a gradual decrease in the sensitivity and an increase in the specificity with the reduction of clinical signs in the dogs that were assessed, reaching a sensitivity of 75% (42.8-94.5%) among asymptomatic dogs and lower specificity of 56% (46.2-66.3%) among symptomatic dogs. Inter-laboratory agreement was substantial (κAB= 0.778; κAC= 0.645; κCB= 0.711). The main conclusion was that the test performance is somewhat dependent on canine symptomatology, but such influence was less evident than in previous studies. Favourable results for sensitivity and specificity can be obtained even in asymptomatic animals; however, caution is needed in these evaluations, and the results suggest that the immunochromatographic test may be further improved for better investigation in asymptomatic dogs. The results obtained confirm the usefulness of DPP® CVL for application in serological surveys. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Prefeitura Municipal de Resende. Vigilância em Saúde. Centro de Controle de Zoonoses. Resende, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Organização Pan-Americana de Saúde. Doenças Negligenciadas, Tropicais e Transmitidas por Vetores. Doenças Transmissíveis e Determinantes Ambientais de Saúde. Duque de Caxias, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Ezequiel Dias. Instituto Otávio Magalhães. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Instituto de Medicina Social. Departamento de Epidemiologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Leishmaniasis | pt_BR |
| Subject | Dual-path platform CVL rapid test | pt_BR |
| Subject | Diagnosis | pt_BR |
| Subject | Dogs | pt_BR |
| Subject in Spanish | Leishmaniasis Visceral | pt_BR |
| Subject in Spanish | Perros | pt_BR |
| DeCS | Leishmaniose Visceral | pt_BR |
| DeCS | Diagnóstico | pt_BR |
| DeCS | Cães | pt_BR |
| e-ISSN | 1678-8060 | |
