| Author | Fonseca, Paula Candida | |
| Author | Nihei, Oscar Kenji | |
| Author | Savino, Wilson | |
| Author | Spray, David C. | |
| Author | Alves, Luiz Anastacio | |
| Access date | 2019-07-04T16:09:04Z | |
| Available date | 2019-07-04T16:09:04Z | |
| Document date | 2006 | |
| Citation | FONSECA, Paula Candida et al. Flow Cytometry Analysis of Gap Junction- Mediated Cell–Cell Communication: Advantages and Pitfalls. Cytometry Part A, v. 69A, p. 487-493, 2006. | pt_BR |
| ISSN | 1552-4922 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/33845 | |
| Language | eng | pt_BR |
| Publisher | Wiley 12 Months | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Citometria de fluxo | pt_BR |
| Subject in Portuguese | Comunicação intracelular | pt_BR |
| Subject in Portuguese | Linfócitos | pt_BR |
| Subject in Portuguese | Junções comunicantes | pt_BR |
| Title | Flow Cytometry Analysis of Gap Junction- Mediated Cell–Cell Communication: Advantages and Pitfalls | pt_BR |
| Type | Article | |
| DOI | 10.1002/cyto.a.20255 | pt_BR |
| Abstract | BACKGROUND: Since the first morphological description of the gap junctions use electron microscopy, a considerable number of techniques has been introduced to evaluate gap junction channel functionality, many of which use dye transfer techniques, such as dye injection and fluorescent dye transfer, analyzed by flow cytometry. METHODS: To analyze dye transfer, generally one population of cells is incubated with calcein-AM (0.5 microM) for 30 min at 37 degrees C, and the other population was incubated with the lipophilic dye DiIC(18) (3) (10 microM) for 1 h at 37 degrees C; after incubation, these cells were washed five times with PBS and cocultured for different times, and then the dye transfer was analyzed by flow cytometry. RESULTS: In this short overview, we focus on some advantages and disadvantages of flow cytometry as a technique to investigate gap junction-mediated intercellular communication (GJIC). In addition, we point out some technical pitfalls that we have encountered when applying this technique to study gap junctions in immune system cells. CONCLUSIONS: Analysis of fluorescent dye transfer by flow cytometry is a useful tool to investigate GJIC. However, some points must be taken into consideration before using this methodology, which are discussed herein. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Comunicação Celular. Rio de Janeiro, RJ. Brasil / Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde, Instituto de Ciências Biomédicas. Programa de Pós-Graduação em Ciências Morfológicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Albert Einstein College of Medicine. Department of Neuroscience. Bronx, NY, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Comunicação Celular. Rio de Janeiro, RJ. Brasil | pt_BR |
| Subject | Gap junctions | pt_BR |
| Subject | Flow cytometry | pt_BR |
| Subject | Intercellular communication | pt_BR |
| Subject | Lymphocytes | pt_BR |
| e-ISSN | 1552-4930 | |
| Embargo date | 2025-01-01 | |
