| Author | Villa-Verde, Déa Maria Serra | |
| Author | Monteiro, Elizangela Silva | |
| Author | Jasiulionis, Miriam G. | |
| Author | Oliveira, Désio Aurélio Farias de | |
| Author | Brentani, Ricardo Renzo | |
| Author | Savino, Wilson | |
| Author | Chammas, Roger | |
| Access date | 2019-07-09T11:08:09Z | |
| Available date | 2019-07-09T11:08:09Z | |
| Document date | 2002 | |
| Citation | VILLA-VERDE, Déa Maria Serra et al. Galectin-3 modulates carbohydrate-dependent thymocyte interactions with the thymic microenvironment. Eur. J. Immunol., v. 32, p. 1434-1444, 2002. | pt_BR |
| ISSN | 0014-2980 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/33897 | |
| Language | eng | pt_BR |
| Publisher | Wiley 12 Months | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Timo | pt_BR |
| Subject in Portuguese | Galectina | pt_BR |
| Subject in Portuguese | Célula Microambiental | pt_BR |
| Subject in Portuguese | Interação célula a célula | pt_BR |
| Subject in Portuguese | Molécula de adesão | pt_BR |
| Title | Galectin-3 modulates carbohydrate-dependent thymocyte interactions with the thymic microenvironment | pt_BR |
| Type | Article | |
| DOI | 10.1002/1521-4141(200205)32:5<1434::AID-IMMU1434>3.0.CO;2-M | pt_BR |
| Abstract | The process of thymocyte differentiation occurs within the context of the thymic microenvironment, in which T cell precursors interact with thymic microenvironmental cells and extracellular matrix. Here we studied the expression of galectin-3, a g -galactoside binding lectin, in the thymus of young adult mice. Galectin-3 was found mainly in the medulla and to a
lesser extent in the cortex. We further showed that distinct microenvironmental elements, such as thymic epithelial cells, the epithelial component of thymic nurse complexes and phagocytic cells of the thymic reticulum produce, secrete and accumulate galectin-3 on the cell surface. Functionally, galectin-3-enriched medium inhibited in vitro thymocyte interactions with thymic microenvironmental cells, accelerated the release of thymocytes from thymic nurse cells and inhibited the reconstitution of these lymphoepithelial complexes. These effects were blocked by exogenous lactose (Gal g 1–4Glc), but not melibiose (Gal § 1–6Glc),
and by a monospecific anti-galectin-3 antibody. Recombinant galectin-3 also inhibited thymocyte/thymic epithelial cell interactions. Our data indicate that intrathymically produced galectin-3 disrupts thymocyte/microenvironmental cell interactions, thus acting as a deadhesion molecule. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil / Instituto Ludwig de Pesquisa sobre o Câncer. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina. Laboratório de Oncologia Experimental. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Instituto Ludwig de Pesquisa sobre o Câncer. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil / Hôpital Necker. Paris, France. | pt_BR |
| Affilliation | Instituto Ludwig de Pesquisa sobre o Câncer. São Paulo, SP, Brasil / Universidade de São Paulo. Faculdade de Medicina. Laboratório de Oncologia Experimental. São Paulo, SP, Brasil. | pt_BR |
| Subject | Thymus | pt_BR |
| Subject | Galectin | pt_BR |
| Subject | Microenvironmental cell | pt_BR |
| Subject | Cell-to-cell interaction | pt_BR |
| Subject | Adhesion molecule | pt_BR |
| e-ISSN | 1521-4141 | |
| Embargo date | 2022-01-01 | |
