| Author | Alves, Lucineia | |
| Author | Lima, Leila de Mendonça | |
| Author | Maeda, Elisa da Silva | |
| Author | Carvalho, Laís | |
| Author | Holy, Jon | |
| Author | Sarno, Euzenir Nunes | |
| Author | Pessolani, Maria Cristina Vidal | |
| Author | Barker, Lucia P. | |
| Access date | 2019-07-12T18:22:41Z | |
| Available date | 2019-07-12T18:22:41Z | |
| Document date | 2004 | |
| Citation | ALVES, Lucineia et al. Mycobacterium leprae infection of human Schwann cells depends on seletive host kinases and pathogen-modulated endocytic pathways. FEMS Microbiology Letters, v. 238, p. 429-437, Aug. 2004. | pt_BR |
| ISSN | 0378-1097 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/34039 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Mycobacterium leprae | pt_BR |
| Subject in Portuguese | Células de Schwann | pt_BR |
| Subject in Portuguese | Fagocitose | pt_BR |
| Subject in Portuguese | Quinases | pt_BR |
| Subject in Portuguese | Macrófagos | pt_BR |
| Subject in Portuguese | Via endocítica | pt_BR |
| Title | Mycobacterium leprae infection of human Schwann cells depends on selective host kinases and pathogen-modulated endocytic pathways | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.femsle.2004.08.007 | |
| Abstract | Mycobacterium leprae, an obligate intracellular pathogen, shows a unique tropism for Schwann cells (SC). This leads to the peripheral neuropathy disorder observed in leprosy. In this study, we investigated signal transduction events and the intracellular fate of M. leprae during the interaction of the microorganism with SC. First, we demonstrated that the human schwannoma cell line ST88-14 readily phagocytized the bacteria as observed by time-lapse microscopy, actin staining and electron microscopy. The effect of specific kinase inhibitors on M. leprae internalization was then investigated showing that functional protein tyrosine kinase, calcium-dependent protein kinase and phosphatidylinositol 3-kinase, but not cAMP-dependent protein kinase are essential for phagocytosis of the bacteria. Similar results were obtained when irradiated and live bacteria were compared and when M. leprae was pre-coated with recombinant histone-like-protein/laminin binding protein, a bacterial adhesin. In addition, experiments were performed to analyze the bacterial trafficking within the endosomal network by labeling the acidified intracellular compartments of M. leprae-infected SC with the Lysotracker acidotrophic probe. Acidification of vesicles containing live M. leprae was minimal in both RAW murine macrophages and SC, although phagosomes containing heat-killed bacteria seem to follow normal endocytic maturation. These data indicate that the invading bacteria interfere with normal endocytic pathway maturation of bacteria-containing phagosomes within SC. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Rio de Janeiro. Departamento de Histologia e Embriologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | University of Minnesota School of Medicine. Department of Medical Microbiology and Immunology. Duluth, MN, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | University of Minnesota School of Medicine. Department of Medical Microbiology and Immunology. Duluth, MN, USA. | pt_BR |
| Subject | Mycobacterium leprae | pt_BR |
| Subject | Schwann cells | pt_BR |
| Subject | Phagocytosis | pt_BR |
| Subject | Kinases | pt_BR |
| Subject | Macrophages | pt_BR |
| Subject | Endocytic pathway | pt_BR |
| Embargo date | 2022-01-01 | |
