| Author | Gomes, Suzete A. O. | |
| Author | Souza, André L. Fonseca de | |
| Author | Silva, Bianca A. | |
| Author | Kiffer-Moreira, Tina | |
| Author | Santos-Mallet, Jacenir R. | |
| Author | Santos, André L. S. | |
| Author | Meyer-Fernandes, José R. | |
| Access date | 2019-07-18T15:58:18Z | |
| Available date | 2019-07-18T15:58:18Z | |
| Document date | 2006 | |
| Citation | GOMES, Suzete A. O. et al. Trypanosoma rangeli: DiVerential expression of cell surface polypeptides and ecto-phosphatase activity in short and long epimastigote forms. Experimental Parasitology, v. 112, p. 253-262, 2006. | pt_BR |
| ISSN | 0014-4894 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/34250 | |
| Language | eng | pt_BR |
| Publisher | Elsevier 12 Months | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Trypanosoma rangeli | pt_BR |
| Subject in Portuguese | Polipeptídeos | pt_BR |
| Subject in Portuguese | Superfície celular | pt_BR |
| Subject in Portuguese | Epimastigotas | pt_BR |
| Subject in Portuguese | Atividade da ecto-fosfatase | pt_BR |
| Title | Trypanosoma rangeli: Differential expression of cell surface polypeptides and ecto-phosphatase activity in short and long epimastigote forms | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.exppara.2005.11.015 | |
| Abstract | Trypanosoma rangeli is a parasite of a numerous wild and domestic animals, presenting wide geographical distribution and high immunological cross-reactivity with Trypanosoma cruzi, which may lead to misdiagnosis. T. rangeli has a complex life cycle, involving distinct morphological and functional forms in the vector. Here, we characterized the cell surface polypeptides and the phosphatase activities in short and long epimastigotes forms of T. rangeli, using intact living parasites. The surface protein profile revealed by the incubation of parasites with biotin showed a preferential expression of the 97, 70, 50, 45, 25-22, and 15 kDa biotinylated polypeptides in the long forms, in contrast to the 55 and 28 kDa biotinylated polypeptides synthesized by the short epimastigotes. Additionally, flow cytometry analysis showed that the short forms had relatively lower biotin surface binding than long ones. The involvement of phosphatases with the trypanosomatid differentiation has been proposed. In this sense, T. rangeli living parasites were able to hydrolyze the artificial substrate p-nitrophenylphosphate at a rate of 25.57+/-2.03 and 10.09+/-0.93 nmol p-NPP x h(-1) x 10(7) cells for the short and long epimastigotes, respectively. These phosphatase activities were linear with time for at least 60 min and the optimum pH lies in the acid range. Classical inhibitors of acid phosphatases, such as ammonium molybdate, sodium fluoride, and zinc chloride, showed a significant decrease in these phosphatase activities, with different patterns of inhibition. Additionally, these phosphatase activities presented different kinetic parameters (Km and Vmax) and distinct sensitivities to divalent cations. Both epimastigotes were unable to release phosphatase to the extracellular environment. Cytochemical analysis demonstrated the localization of these enzymes on the parasite surfaces (cell body and flagellum) and in intracellular vacuoles, resembling acidocalcisomes. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Bioquímica Médica. Laboratório de Bioquímica Celular.. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Bioquímica Médica. Laboratório de Bioquímica Celular.. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Laboratório de Estudos Integrados em Bioquímica Microbiana. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Bioquímica Médica. Laboratório de Bioquímica Celular.. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Entomologia. Núcleo de Morfologia e Ultraestrutura. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Laboratório de Estudos Integrados em Bioquímica Microbiana. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Bioquímica Médica. Laboratório de Bioquímica Celular.. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Trypanosoma rangeli | pt_BR |
| Subject | Cell surface polypeptides | pt_BR |
| Subject | Ecto-phosphatase activity | pt_BR |
| Subject | Epimastigote | pt_BR |
| e-ISSN | 1090-2449 | |
| Embargo date | 2022-01-01 | |
