| Author | Paredes, Bruno Diaz | |
| Author | Martins, Gabriele Louise Soares | |
| Author | Azevedo, Carine Machado | |
| Author | Sampaio, Gabriela Louise de Almeida | |
| Author | Nonaka, Carolina Kymie Vasques | |
| Author | Silva, Katia Nunes da | |
| Author | Soares, Milena Botelho Pereira | |
| Author | Santos, Ricardo Ribeiro dos | |
| Author | Souza, Bruno Solano de Freitas | |
| Access date | 2019-07-31T17:21:24Z | |
| Available date | 2019-07-31T17:21:24Z | |
| Document date | 2019 | |
| Citation | PAREDES, Bruno Diaz et al. Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies. Stem Cell Research, v. 38, p. 1-5, 2019. | pt_BR |
| ISSN | 1873-5061 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/34544 | |
| Sponsorship | CNPq, CAPES, FAPESB, Ministry of Health, National Induced Pluripotent Stem Cell Biobank and the National Institute of Science and Technology for Regenerative Medicine. | pt_BR |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Doença Falciforme | pt_BR |
| Subject in Portuguese | Hemoglobinopatias | pt_BR |
| Subject in Portuguese | Celulas IPS | pt_BR |
| Title | Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.scr.2019.101454 | |
| Abstract | Sickle cell disease (SCD) is one of the most prevalent and severe monogenetic disorders. Previously, we generated iPS cell lines from SCD patients. Here, we generated iPS cell lines from three age-, ethnicity- and gender-matched healthy individuals as control cell lines. Cell reprogramming was performed using erythroblasts expanded from PBMC by a non-integrative method. SCD-iPSC controls expressed pluripotency markers, presented a normal karyotype, were able to differentiate into the three germ layers in embryoid body spontaneous differentiation and confirmed to be integration-free. The cell lines generated here may be used as matched healthy controls for SCD studies. | pt_BR |
| Affilliation | São Rafael Hospital. D'Or Institute for Research and Education. Salvador, BA, Brazil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil. | pt_BR |
| Affilliation | São Rafael Hospital. D'Or Institute for Research and Education. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | São Rafael Hospital. D'Or Institute for Research and Education. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | São Rafael Hospital. D'Or Institute for Research and Education. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | São Rafael Hospital. D'Or Institute for Research and Education. Salvador, BA, Brazil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. D'Or Institute for Research and Education. Salvador, BA, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. D'Or Institute for Research and Education. Salvador, BA, Brazil. | pt_BR |
| Affilliation | São Rafael Hospital. D'Or Institute for Research and Education. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. D'Or Institute for Research and Education. Salvador, BA, Brazil. | pt_BR |
| Subject | Anemia Sickle Cell | pt_BR |
| Subject | Hemoglobinopathies | pt_BR |
| Subject | Induced Pluripotent Stem Cells | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
