Author | Daltro, Ramona Tavares | |
Author | Leony, Leonardo Maia | |
Author | Freitas, Natália Erdens Maron | |
Author | Silva, Ângelo Antônio Oliveira | |
Author | Santos, Emily Ferreira | |
Author | Del-Rei, Rodrigo Pimenta | |
Author | Brito, Maria Edileuza Felinto | |
Author | Brandão Filho, Sinval Pinto | |
Author | Gomes, Yara Miranda | |
Author | Silva, Marcelo Sousa | |
Author | Donato, Silvia Tavares | |
Author | Jeronimo, Selma Maria Bezerra | |
Author | Monteiro, Gloria Regina de Góis | |
Author | Carvalho, Lucas Pedreira | |
Author | Magalhães, Andréa Santos | |
Author | Zanchin, Nilson Ivo Tonin | |
Author | Celedon, Paola Alejandra Fiorani | |
Author | Santos, Fred Luciano Neves | |
Access date | 2019-08-01T14:02:44Z | |
Available date | 2019-08-01T14:02:44Z | |
Document date | 2019 | |
Citation | DALTRO, Ramona Tavares et al. Cross-Reactivity Using Chimeric Trypanosoma cruzi Antigens: Diagnostic Performance in Settings Where Chagas Disease and American Cutaneous or Visceral Leishmaniasis Are Coendemic. Journal of Clinical Microbiology, v. 57, n. 8, p. 1-10, 2019. | pt_BR |
ISSN | 0095-1137 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/34560 | |
Description | O artigo encontra-se disponível em acesso aberto no site do Editor. | pt_BR |
Sponsorship | FIOCRUZ Technical Platform program for providing antigen
purification infrastructure. Internal Research Support Program–PIAP Jovem
(grant PIAP-001/2017); the Coordination of Superior Level Staff Improvement–CAPES
(grant PROEX 0720/2018), the National Council for Scientific and Technological Development–
CNPq (grants 479046/2011-5 and 400331/2012-8), Araucária Foundation
(grant 05/2016), and Research Support Foundation of the State of Bahia (FAPESB). | pt_BR |
Language | eng | pt_BR |
Publisher | American Society for Microbiology | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leishmaniose tegumentar americana | pt_BR |
Subject in Portuguese | Doença de Chagas | pt_BR |
Subject in Portuguese | Reatividade cruzada | pt_BR |
Subject in Portuguese | Antígenos quiméricos recombinantes | pt_BR |
Subject in Portuguese | Leishmaniose visceral | pt_BR |
Title | Cross-Reactivity Using Chimeric Trypanosoma cruzi Antigens: Diagnostic Performance in Settings Where Chagas Disease and American Cutaneous or Visceral Leishmaniasis Are Coendemic | pt_BR |
Type | Article | pt_BR |
DOI | 10.1128/JCM.00762-19 | |
Abstract | Chimeric T. cruzi antigens have been proposed as a diagnostic tool for chronic Chagas disease (CD) in both settings where Chagas disease is endemic and those where it is not endemic. Antibody response varies in accordance to each T. cruzi strain, presenting challenges to the use of antigens lacking demonstrated cross-reactivity with Leishmania spp. Our group expressed four chimeric proteins (IBMP-8.1, IBMP-8.2, IBMP-8.3, and IBMP-8.4) and previously assessed their diagnostic performance to determine cross-reactivity with Leishmania spp. Here, we validated our findings using serum samples from different Brazilian geographic areas reporting endemic Chagas disease, endemic visceral or American cutaneous leishmaniasis (ACL), or both. Overall, 829 serum samples were evaluated using commercial and IBMP enzyme-linked immunosorbent assays. Due to the absence of a reference assay to diagnosis CD, latent class analysis (LCA) was performed through the use of a statistical model. The incidence of cross-reactivity for ACL-positive samples varied from 0.35% (IBMP-8.3) to 0.70% (IBMP-8.1 and IBMP-8.2). Regarding visceral leishmaniasis (VL)-positive samples, the IBMP-8.2 and IBMP-8.3 antigens cross-reacted with six (3.49%) and with only one sample (0.58%), respectively. No cross-reactivity with either ACL or VL was observed for the IBMP-8.4 antigen. Similarly, no cross-reactions were found when VL-positive samples were assayed with IBMP-8.1. The agreement among the results obtained using IBMP antigens ranged from 97.3% for IBMP-8.2 and 99% for IBMP-8.1 and IBMP-8.3 to 100% for IBMP-8.4, demonstrating almost perfect agreement with LCA. Accordingly, in light of the negligible cross-reactivity with both ACL and VL, we suggest the use of IBMP antigens in regions where T. cruzi and Leishmania spp. are coendemic. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Faculty of Technology and Sciences of Bahia. Salvador, BA, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
Affilliation | Federal University of Rio Grande do Norte. Natal, RN, Brazil / Institute of Hygiene and Tropical Medicine. Lisbon, Portugal. | pt_BR |
Affilliation | Federal University of Rio Grande do Norte. Natal, RN, Brazil. | pt_BR |
Affilliation | Federal University of Rio Grande do Norte. Natal, RN, Brazil. | pt_BR |
Affilliation | Federal University of Rio Grande do Norte. Natal, RN, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Salvador, BA, Brazil. | pt_BR |
Affilliation | Federal University of Bahia. Salvador, BA, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Molecular Biology Institute of Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Subject | American cutaneous leishmaniasis | pt_BR |
Subject | Chagas disease | pt_BR |
Subject | Cross-reactivity | pt_BR |
Subject | Recombinant chimeric antigens | pt_BR |
Subject | Visceral leishmaniasis | pt_BR |
Embargo date | 2020-01-06 | |