| Author | Yanasse, Ricardo Hideki | |
| Author | Lábio, Roger William de | |
| Author | Marques, Leonardo | |
| Author | Fukasawa, Josianne Tomazini | |
| Author | Segato, Rosimeire | |
| Author | Kinoshita, Angela | |
| Author | Matsumoto, Mariza Akemi | |
| Author | Felisbino, Sergio Luis | |
| Author | Souza, Bruno Solano de Freitas | |
| Author | Santos, Ricardo Ribeiro dos | |
| Author | Payão, Spencer Luiz Marques | |
| Access date | 2019-08-07T13:38:37Z | |
| Available date | 2019-08-07T13:38:37Z | |
| Document date | 2019 | |
| Citation | YANASSE, Ricardo Hideki et al. Xenotransplantation of human dental pulp stem cells in platelet-rich plasma for the treatment of full-thickness articular cartilage defects in a rabbit model. Experimental and Therapeutic Medicine, v. 17, p. 4344-4356, 2019. | pt_BR |
| ISSN | 1792-0981 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/34648 | |
| Description | Santos, Ricardo Ribeiro dos; Souza Bruno Solano de Freitas. Fundação Oswaldo Cruz. Instituto Gonçalo Muniz. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento. | pt_BR |
| Sponsorship | São Paulo Research Foundation (FAPESP) (grant no. 12/21007‑0). | pt_BR |
| Language | eng | pt_BR |
| Publisher | Spandidos Publications | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Cartilagem | pt_BR |
| Subject in Portuguese | Polpa dentária | pt_BR |
| Subject in Portuguese | Joelho | pt_BR |
| Subject in Portuguese | Plasma rico em plaquetas | pt_BR |
| Subject in Portuguese | Células estaminais | pt_BR |
| Title | Xenotransplantation of human dental pulp stem cells in platelet-rich plasma for the treatment of full-thickness articular cartilage defects in a rabbit model | pt_BR |
| Type | Article | |
| DOI | 10.3892/etm.2019.7499 | |
| Abstract | Stem cells in platelet-rich plasma (PRP) scaffolds may be a promising treatment for cartilage repair. Human dental pulp stem cell (hDPSC) subpopulations have been identified to have substantial angiogenic, neurogenic and regenerative potential when compared with other stem cell sources. The present study evaluated the potential of hDPSCs in a PRP scaffold to regenerate full-thickness cartilage defects in rabbits. Full-thickness articular cartilage defects were created in the patellar groove of the femur of 30 rabbits allocated into three experimental groups: Those with an untreated critical defect (CTL), those treated with PRP (PRP) and those treated with stem cells in a PRP scaffold (PRP+SC). The patellar grooves of the femurs from the experimental groups were evaluated macroscopically and histologically at 6 and 12 weeks post-surgery. The synovial membranes were also collected and evaluated for histopathological analysis. The synovial lining cell layer was enlarged in the CTL group compared with the PRP group at 6 weeks (P=0.037) but not with the PRP+SC group. All groups exhibited low-grade synovitis at 6 weeks and no synovitis at 12 weeks. Notably, macroscopic grades for the area of articular cartilage repair for the PRP+SC group were significantly improved compared with those in the CTL (P=0.001) and PRP (P=0.049) groups at 12 weeks. Furthermore, histological scores (modified O'Driscoll scoring system) of the patellar groove articular cartilage in the PRP+SC and PRP groups, in which the articular cartilage was primarily hyaline-like, were significantly higher compared with those in the CTL group at 12 weeks (P=0.002 and P=0.007, respectively). The present results support the therapeutic use of hDPSCs for the treatment of full-thickness articular cartilage defects. | pt_BR |
| Affilliation | Faculdade de Medicina de Marília. Department of Genetics. Blood Center. Marília, SP, Brasil. | pt_BR |
| Affilliation | Faculdade de Medicina de Marília. Department of Genetics. Blood Center. Marília, SP, Brasil. | pt_BR |
| Affilliation | Universidade do Sagrado Coração. Department of Health Sciences. Bauru, SP, Brasil. | pt_BR |
| Affilliation | Faculdade de Medicina de Marília. Department of Genetics. Blood Center. Marília, SP, Brasil. | pt_BR |
| Affilliation | Faculdade de Medicina de Marília. Department of Genetics. Blood Center. Marília, SP, Brasil. | pt_BR |
| Affilliation | Universidade do Sagrado Coração. Department of Health Sciences. Bauru, SP, Brasil. | pt_BR |
| Affilliation | Universidade do Sagrado Coração. Department of Health Sciences. Bauru, SP, Brasil. | pt_BR |
| Affilliation | Universidade Estadual Paulista Júlio de Mesquita Filho. Department of Morphology. Botucatu, SP, Brazil. | pt_BR |
| Affilliation | Monte Tabor Hospital São Rafael. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil. | pt_BR |
| Affilliation | Monte Tabor Hospital São Rafael. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil. | pt_BR |
| Affilliation | Faculdade de Medicina de Marília. Department of Genetics. Blood Center. Marília, SP, Brasil / Universidade do Sagrado Coração. Department of Health Sciences. Bauru, SP, Brasil. | pt_BR |
| Subject | Cartilage | pt_BR |
| Subject | Dental pulp | pt_BR |
| Subject | Knee | pt_BR |
| Subject | Platelet‑rich plasma | pt_BR |
| Subject | Stem cells | pt_BR |
