| Author | Carvalho, Gustavo Mayr de Lima | |
| Author | Rego, Felipe Dutra | |
| Author | Tanure, Aline | |
| Author | Silva, A. C. P. | |
| Author | Dias, Thaís Alvares | |
| Author | Paz, Gustavo Fontes | |
| Author | Andrade Filho, Jose Dilermando | |
| Access date | 2019-08-13T14:14:24Z | |
| Available date | 2019-08-13T14:14:24Z | |
| Document date | 2017 | |
| Citation | Carvalho, Gustavo Mayr de Lima et al. Bloodmeal Identification in Field-Collected Sand Flies From Casa Branca, Brazil, Using the Cytochrome b PCR Method. Journal of Medical Entomology, v. 54, n. 4, p. 1049-1054, 2017. | pt_BR |
| ISSN | 0022-2585 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/34768 | |
| Language | eng | pt_BR |
| Publisher | Oxford University Press | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Bloodmeal Identification in Field-Collected Sand Flies From Casa Branca, Brazil, Using the Cytochrome b PCR Method | pt_BR |
| Type | Article | |
| DOI | 10.1093/jme/tjx051 | |
| Abstract | PCR-based identification of vertebrate host bloodmeals has been performed on several vectors species with success. In the present study, we used a previously published PCR protocol followed by DNA sequencing based on primers designed from multiple alignments of the mitochondrial cytochrome b gene used to identify avian and mammalian hosts of various hematophagous vectors. The amplification of a fragment encoding a 359 bp sequence of the Cyt b gene yielded recognized amplification products in 192 female sand flies (53%), from a total of 362 females analyzed. In the study area of Casa Branca, Brazil, blood-engorged female sand flies such as Lutzomyia longipalpis (Lutz & Neiva, 1912), Migonemyia migonei (França, 1924), and Nyssomyia whitmani (Antunes & Coutinho, 1939) were analyzed for bloodmeal sources. The PCR-based method identified human, dog, chicken, and domestic rat blood sources. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil. | pt_BR |
| Subject | Bloodmeal identification | pt_BR |
| Subject | Cytochrome b PCR | pt_BR |
| Subject | Sand fly | pt_BR |
| Embargo date | 2030-01-01 | |
