| Author | Hojo-Souza, Natália Satchiko | |
| Author | Pereira, Dhelio Batista | |
| Author | Passos, Lívia Silva Araújo | |
| Author | Gazzinelli-Guimarães, Pedro Henrique | |
| Author | Cardoso, Mariana Santos | |
| Author | Tada, Mauro Shugiro | |
| Author | Zanini, Graziela Maria | |
| Author | Bartholomeu, Daniella Castanheira | |
| Author | Fujiwara, Ricardo Toshio | |
| Author | Bueno, Lilian Lacerda | |
| Access date | 2019-08-13T14:21:37Z | |
| Available date | 2019-08-13T14:21:37Z | |
| Document date | 2015 | |
| Citation | HOJO-SOUZA, Natália Satchiko et al. Phenotypic profiling of CD8+ T cells during Plasmodium vivax blood-stage infection. BMC Infectious Diseases, v. 15, p. 1-9, Jan. 2015. | pt_BR |
| ISSN | 1471-2334 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/34769 | |
| Language | eng | pt_BR |
| Publisher | BioMed Central | pt_BR |
| Rights | open access | pt_BR |
| Title | Phenotypic profiling of CD8+ T cells during Plasmodium vivax blood-stage infection | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1186/s12879-015-0762-x | |
| Abstract | Background: For a long time, the role of CD8(+) T cells in blood-stage malaria was not considered important because erythrocytes do not express major histocompatibility complex (MHC) class I proteins. While recent evidences suggest that CD8(+) T cells may play an important role during the erythrocytic phase of infection by eliminating parasites, CD8(+) T cells might also contribute to modulate the host response through production of regulatory cytokines. Thus, the role of CD8(+) T cells during blood-stage malaria is unclear. Here, we report the phenotypic profiling of CD8(+) T cells subsets from patients with uncomplicated symptomatic P. vivax malaria. Methods: Blood samples were collected from 20 Plasmodium vivax-infected individuals and 12 healthy individuals. Immunophenotyping was conducted by flow cytometry. Plasma levels of IFN-γ, TNF-α and IL-10 were determined by ELISA/CBA. Unpaired t-test or Mann-Whitney test was used depending on the data distribution. Results: P. vivax-infected subjects had lower percentages and absolute numbers of CD8(+)CD45RA(+) and CD8(+)CD45RO(+) T cells when compared to uninfected individuals (p ≤ 0.0002). A significantly lower absolute number of circulating CD8(+)CD45(+)CCR7(+) cells (p = 0.002) was observed in P. vivax-infected individuals indicating that infection reduces the number of central memory T cells. Cytokine expression was significantly reduced in the naïve T cells from infected individuals compared with negative controls, as shown by lower numbers of IFN-γ(+) (p = 0.001), TNF-α(+) (p < 0.0001) and IL-10(+) (p < 0.0001) CD8(+) T cells. Despite the reduction in the number of CD8(+) memory T cells producing IFN-γ (p < 0.0001), P. vivax-infected individuals demonstrated a significant increase in memory CD8(+)TNF-α(+) (p = 0.016) and CD8(+)IL-10(+) (p = 0.004) cells. Positive correlations were observed between absolute numbers of CD8(+)IL-10(+) and numbers of CD8(+)IFN-γ(+) (p < 0.001) and CD8(+)TNF-α(+) T cells (p ≤ 0.0001). Finally, an increase in the plasma levels of TNF-α (p = 0.017) and IL-10 (p = 0.006) and a decrease in the IFN-γ plasma level (p <0.0001) were observed in the P. vivax-infected individuals. Conclusions: P. vivax infection reduces the numbers of different subsets of CD8(+) T cells, particularly the memory cells, during blood-stage of infection and enhances the number of CD8(+) memory T cells expressing IL-10, which positively correlates with the number of cells expressing TNF-α and IFN-γ. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro. RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Subject | Plasmodium vivax | pt_BR |
| Subject | Malaria | pt_BR |
| Subject | CD8+ T lymphocytes | pt_BR |
| e-ISSN | 1471-2334 | |
