Author | Wippel, Helisa Helena | |
Author | Malgarin, Juliane Soldi | |
Author | Inoue, Alexandre Haruo | |
Author | Leprevost, Felipe da Veiga | |
Author | Carvalho, Paulo Costa | |
Author | Goldenberg, Samuel | |
Author | Alves, Lysangela Ronalte | |
Access date | 2019-08-15T19:23:11Z | |
Available date | 2019-08-15T19:23:11Z | |
Document date | 2019 | |
Citation | WIPPEL, Helisa Helena et al. Unveiling the partners of the DRBD2-mRNP complex, an RBP in Trypanosoma cruzi and ortholog to the yeast SR-protein Gbp2. BMC Microbiology, v. 19, n. 128, p. 1-12, 2019. | pt_BR |
ISSN | 1471-2180 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/34842 | |
Language | por | pt_BR |
Publisher | BMC | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Metabolismo de RNA | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Title | Unveiling the partners of the DRBD2-mRNP complex, an RBP in Trypanosoma cruzi and ortholog to the yeast SR-protein Gbp2 | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/s12866-019-1505-8 | |
Abstract | RNA-binding proteins (RBPs) are well known as key factors in gene expression regulation in eukaryotes. These proteins associate with mRNAs and other proteins to form mRNP complexes that ultimately determine the fate of target transcripts in the cell. This association is usually mediated by an RNA-recognition motif (RRM). In the case of trypanosomatids, these proteins play a paramount role, as gene expression regulation is mostly posttranscriptional. Despite their relevance in the life cycle of Trypanosoma cruzi, the causative agent of
Chagas’ disease, to date, few RBPs have been characterized in this parasite. And as results , we investigated the role of DRBD2 in T. cruzi, an RBP with two RRM domains that is associated with cytoplasmic translational complexes. We show that DRBD2 is an ortholog of the Gbp2 in yeast, an SR-rich protein involved in mRNA quality control and export. We used an immunoprecipitation assay followed by shotgun proteomics and RNA-seq to assess the interaction partners of the DRBD2-mRNP complex in epimastigotes. The analysis identified mostly proteins involved in RNA metabolism and regulation, such as ALBA1, ALBA3, ALBA4, UBP1, UBP2, DRBD3, and PABP2. The RNA-seq results showed that most of the transcripts regulated by the DRBD2
complex mapped to hypothetical proteins related to multiple processes, such as to biosynthetic process, DNA
metabolic process, protein modification, and response to stress. Thus, It concluded that, the identification of regulatory proteins in the DRBD2-mRNP complex corroborates the important role of DRBD2 in gene expression regulation in T. cruzi. We consider these results an important contribution to future studies regarding gene expression regulation in T. cruzi, especially in the field of RNA-binding proteins. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas.Curitiba, PR, Brasil. | pt_BR |
Affilliation | Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas.Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Medical Science Unit I. Department of Pathology. University of Michigan. Michigan, USA. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas.Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas.Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Subject | RNA-Binding Proteins | pt_BR |
Subject | Gene Expression Regulation | pt_BR |
Subject | RNA | pt_BR |
Subject in Spanish | Proteínas de Unión al ARN | pt_BR |
Subject in Spanish | Regulación de la Expresión Génica | pt_BR |
Subject in Spanish | ARN | pt_BR |
DeCS | Proteínas de Ligação a RNA | pt_BR |
DeCS | Regulação da Expressão Gênica | pt_BR |
DeCS | RNA | pt_BR |
e-ISSN | 1471-2180 | |