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AutorBanu, Sultana Shahana
AutorMeyer, Wieland
AutorFerreira-Paim, Kennio
AutorWang, Qinning
AutorKuhls, Katrin
AutorCupolillo, Elisa
AutorSchönian, Gabriele
AutorLee, Rogan
Fecha de acceso2019-08-23T18:37:39Z
Fecha de disponibilización2019-08-23T18:37:39Z
Fecha de publicación2019
ReferenciaBANU, Sultana Shahana et al. A novel multilocus sequence typing scheme identifying genetic diversity amongst Leishmania donovani isolates from a genetically homogeneous population in the Indian subcontinent. International Journal for Parasitology, v. 49, n. 7, p. 555-567, June 2019.pt_BR
ISSN0020-7519pt_BR
URIhttps://www.arca.fiocruz.br/handle/icict/35045
Idiomaengpt_BR
EditorElsevier 12 Monthspt_BR
Derechos de autorrestricted access
Palabras clave en PortuguésLeishmania donovanipt_BR
Palabras clave en PortuguésDiversidade genéticapt_BR
Palabras clave en PortuguésDigitação de seqüência de foco múltiplopt_BR
Palabras clave en PortuguésSubcontinente indianopt_BR
Palabras clave en PortuguésBangladeshpt_BR
TítuloA novel multilocus sequence typing scheme identifying genetic diversity amongst Leishmania donovani isolates from a genetically homogeneous population in the Indian subcontinentpt_BR
Tipo del documentoArticle
DOI10.1016/j.ijpara.2019.02.010
Resumen en InglésIn the Indian subcontinent, infection with Leishmania donovani can cause fatal visceral leishmaniasis. Genetic variation in L. donovani is believed to occur rapidly from environmental changes and through selective drug pressures, thereby allowing continued disease occurrence in this region. All previous molecular markers that are commonly in use multilocus microsatellite typing and multilocus sequence typing, were monomorphic in L. donovani originating from the Indian subcontinent (with only a few exceptions) and hence are not suitable for this region. An multilocus sequence typing scheme consisting of a new set of seven housekeeping genes was developed in this study, based on recent findings from whole genome sequencing data. This new scheme was used to assess the genetic diversity amongst 22 autochthonous L. donovani isolates from Bangladesh. Nineteen additional isolates of the L. donovani complex (including sequences of L. donovani reference strain BPK282A1) from other countries were included for comparison. By using restriction fragment length polymorphism of the internal transcribed spacer 1 region (ITS1-RFLP) and ITS1 sequencing, all Bangladeshi isolates were confirmed to be L. donovani. Population genetic analyses of 41 isolates using the seven new MLST loci clearly separated L. donovani from Leishmania infantum. With this multilocus sequence typing scheme, seven genotypes were identified amongst Bangladeshi L. donovani isolates, and these isolates were found to be phylogenetically different compared with those from India, Nepal, Iraq and Africa. This novel multilocus sequence typing approach can detect intra- and inter-species variations within the L. donovani complex, but most importantly these molecular markers can be applied to resolve the phylogenetically very homogeneous L. donovani strains from the Indian subcontinent. Four of these markers were found suitable to differentiate strains originating from Bangladesh, with marker A2P being the most discriminative one.pt_BR
AfiliaciónParasitology Department. Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research. Westmead Hospital. Westmead, Sydney, NSW, Australia / Molecular Mycology Research laboratory. Centre for Infectious Diseases and Microbiology. Faculty of Medicine and Health. Sydney Medical School. Westmead Clinical School, Marie Bashir Institut for Emerging Infectious Diseases and Biosecurity. Westmead Hospital (Research and Education Network), The University of Sydney. Sydney, NSW, Australia / Westmead Institute for Medical Research. Sydney, NSW, Australia / Directorade General of health Services, Ministry of Health and family Welfare. Dhaka, Bangladesh.pt_BR
AfiliaciónMolecular Mycology Research Laboratory. Centre for Infectious Diseases and Microbiology. Faculty of Medicine and Health. Sydney Medical School. Westmead Clinical School, Marie Bashir Institut for Emerging Infectious Diseases and Biosecurity. Westmead Hospital (Research and Education Network), The University of Sydney. Sydney, NSW, Australia / Westmead Institute for Medical Research. Sydney, NSW, Australia.pt_BR
AfiliaciónMolecular Mycology Research Laboratory. Centre for Infectious Diseases and Microbiology. Faculty of Medicine and Health. Sydney Medical School. Westmead Clinical School, Marie Bashir Institut for Emerging Infectious Diseases and Biosecurity. Westmead Hospital (Research and Education Network), The University of Sydney. Sydney, NSW, Australia / Westmead Institute for Medical Research. Sydney, NSW, Australia / Universidade Federal do Triângulo Mineiro. Departamento de Microbiologia. Uberaba, MG, Brasil.pt_BR
AfiliaciónParasitology Department. Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research. Westmead Hospital. Westmead, Sydney, NSW, Australia.pt_BR
AfiliaciónDivision of Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Widau. Widau, Germany.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónInstitute for Microbiology and Hygiene CC05, Charité University of Medicine Berlin. Berlin, Germany.pt_BR
AfiliaciónParasitology Department. Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research. Westmead Hospital. Westmead, Sydney, NSW, Australia / Molecular Mycology Research laboratory. Centre for Infectious Diseases and Microbiology. Faculty of Medicine and Health. Sydney Medical School. Westmead Clinical School, Marie Bashir Institut for Emerging Infectious Diseases and Biosecurity. Westmead Hospital (Research and Education Network), The University of Sydney. Sydney, NSW, Australia.pt_BR
Palavras clave en InglêsLeishmania donovanipt_BR
Palavras clave en InglêsGenetic diversitypt_BR
Palavras clave en InglêsMultilocus sequence typingpt_BR
Palavras clave en InglêsIndian subcontinentpt_BR
Palavras clave en InglêsBangladeshpt_BR
e-ISSN1879-0135
Fecha de embargo2025-01-01
xmlui.metadata.dc.subject.ods10 Redução das desigualdades


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