| Author | Pinheiro, Maria Letícia B. | pt_BR |
| Author | Feres-Filho, Eduardo J. | pt_BR |
| Author | Graves, Dana T. | pt_BR |
| Author | Takiya, Christina M. | pt_BR |
| Author | Elsas, Maria Ignez Capella Gaspar | pt_BR |
| Author | Xavier-Elsas, Pedro Paulo | pt_BR |
| Author | Luz, Ricardo A. | pt_BR |
| Access date | 2010-08-23T16:00:28Z | |
| Available date | 2010-08-23T16:00:28Z | |
| Document date | 2003 | |
| Citation | PINHEIRO, Maria Letícia B. et al. Quantification and localization of Platelet-derived growth factor in gingiva of periodontitis patients. J. Periodontol, Indianapolis, v. 74, p. 323-328, 2003. | pt_BR |
| ISSN | 0022-3492 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/352 | |
| Language | eng | pt_BR |
| Rights | restricted access | |
| Title | Quantification and localization of Platelet-derived growth factor in gingiva of periodontitis patients | pt_BR |
| Type | Article | |
| Abstract | Platelet-derived growth factor (PDGF) is a mitogen and chemoattractant for cells of mesenchymal origin. Overexpression of PDGF-B can promote formation of inflammatory lesions in the lungs of transgenic mice. Moreover, continuous exposure to PDGF inhibits collagen production by osteoblastic cells. Thus, the expression of mitogenic factors in an inflammatory context may limit the differentiated function of cells, and thereby limit repair following periodontal attachment and bone loss. The goals of the present study were to test whether PDGF is present at increased levels in inflamed gingiva and to localize its expression in gingival biopsies from individuals with chronic periodontitis. Methods: Tissues obtained during therapeutic procedures from inflamed and control sites of 9 patients were subjected to protein extraction, descriptive histology by hematoxylin and eosin, or immunohistochemistry assays. Quantification was calculated with an enzyme-linked immunosorbent assay (ELISA) kit specific for PDGF-AB. For the immunolocalization, antiPDGF-A and -B antibodies were employed. Results: PDGF concentration in the total protein extract was approximately 3 times higher in the inflamed sites (0.60 0.18 ng/mg versus 0.20 0.05 ng/mg; P= 0.03). Immunohistochemistry revealed prominent expression of PDGF in the pocket epithelial cells as well as the adjacent connective tissue. In contrast, little or no expression was detected in control biopsies devoid of the pocket epithelium and granulation tissue. Conclusions: PDGF is present in increased levels in the human inflamed gingiva and is mainly localized to the pocket epithelium. It is possible that chronic expression of PDGF contributes to the inflammatory changes that occur during periodontal diseases. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Faculdade de Odontologia. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Faculdade de Odontologia. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Boston University. School of Dental Medicine. Departament of Periodontology. Boston, USA. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Departamento de Histologia e Embriologia. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade do Rio de Janeiro. Instituto de Microbiologia. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | |
| Subject | Gingiva | |
| Subject | Platelet-derived | |
| Subject | Inflammatory Response | |
| Subject | Periodontitis | |
| Subject | Growth Factors | |
| DeCS | Gengiva - fisiologia | |
| DeCS | Peptídeos e Proteínas de Sinalização Intercelular | |
| DeCS | Fator de Crescimento Derivado de Plaquetas | |
| DeCS | Periodontite - fisiologia | |
