| Author | Santoro, Tassia | |
| Author | Azevedo, Carolina T. | |
| Author | Silva, Patrícia M. R. e | |
| Author | Martins, Marco A. | |
| Author | Carvalho, Vinicius F. | |
| Access date | 2019-09-03T16:14:16Z | |
| Available date | 2019-09-03T16:14:16Z | |
| Document date | 2019 | |
| Citation | SANTORO, Tassia et al. Glucocorticoids decrease the numbers and activation ofmast cells by inducing the transactivation receptors of AGEs. Journal of Leukocyte Biology, v. 105, p. 131-142, 2019. | pt_BR |
| ISSN | 0741-5400 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/35317 | |
| Language | eng | pt_BR |
| Publisher | Wiley 12 Months | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Alergia | pt_BR |
| Subject in Portuguese | Mastócitos | pt_BR |
| Subject in Portuguese | Apoptose | pt_BR |
| Title | Glucocorticoids decrease the numbers and activation of mast cells by inducing the transactivation receptors of AGEs | pt_BR |
| Type | Article | |
| DOI | 10.1002/JLB.3A0917-364RR | |
| Abstract | Glucocorticoids (GCs) are potent anti-allergic compounds that function, at least in part, by inhibiting signaling pathways in mast cells. We hypothesized that the GC-induced mastocytopenia and suppression of mast cell activation are mediated by the advanced glycation end products (AGEs)/receptors of AGEs (RAGEs) signaling axis. We evaluated the role of AGEs in GC-mediated mastocytopenia and impaired mast cell degranulation in male Wistar rats and Swiss-Webster mice subcutaneously injected with dexamethasone or prednisolone (0.1 mg/kg) once a day for 21 consecutive days. The animals were treated with either the AGE inhibitor aminoguanidine (250 mg/kg), the RAGE antagonist FPS-ZM1 (1 mg/kg) or the galectin-3 antagonist GSC-100 (1 mg/kg) daily for 18 days, starting 3 days following GC treatment. Aminoguanidine inhibited GC-induced mast cell apoptosis and restored mast cell numbers in the pleural cavity of GC-treated rats. Aminoguanidine also reversed the GC-induced reduction in histamine release triggered by allergens or compound 48/80 in vitro. GC treatment induced RAGE and galectin expression in mast cells, and blocking these agents by FPS-ZM1 or GSC-100 significantly reversed mast cell numbers in the peritoneal cavity and mesenteric tissue of GC-treated mice. In addition, the combination of GC and AGE-induced mast cell apoptosis in vitro was inhibited by both FPS-ZM1 and GSC-100. We concluded that the GC-induced mastocytopenia and suppression of mast cell stimulation are associated with the gene transactivation of RAGE and galectin-3. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimmunomodulação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Mast cells | pt_BR |
| Subject | Allergy | pt_BR |
| Subject | Apoptosis | pt_BR |
| e-ISSN | 1938-3673 | |
| Embargo date | 2025-01-01 | |
